Controlling S-phase Onset in Fission Yeast
- Z. LYGEROU and
- P. NURSE
This extract was created in the absence of an abstract.
Excerpt
To preserve the integrity of the cell's genome, DNAreplication (S phase) must alternate with mitosis (Mphase) every cell cycle, and the genome must be duplicated entirely and only once at each S phase. To replicateits entire 14 Mb of genomic DNA within the 10 minutesthat an average S-phase lasts, a fission yeast cell needs toactivate hundreds of origins of DNA replication. To prevent mitosis from taking place until S phase is completed,control mechanisms have to monitor whether all activeorigins have fired and every part of the genome has beenreplicated. At the same time, they must inhibit originsfrom firing a second time or a replication fork from goingthrough a region of the genome that has already beenreplicated. The control mechanisms that regulate thiscomplex set of events have been subject to considerablestudy during the past decade, and substantial progress inour understanding has resulted from the combined effortsof many laboratories working on various model systems.We present here recent data on the controls acting over Sphase onset in the fission yeast Schizosaccharomycespombe and discuss how they relate to data from othermodel systems...
