Abstract
Background/Aim: During screening for compounds that selectively suppress growth of human colorectal cancer (CRC) spheroids with mutant (mt) KRAS, the uridine analogue, 5-bromouridine (BrUrd) was identified and its derivatives were explored. Materials and Methods: DNA incorporation in two-dimensional (2D) and three-dimensional floating (3DF) cultures was examined with the uridine analogue, 5-ethynyl-2’-deoxyuridine (EdU). The area of HKe3 CRC spheroids expressing wild type (wt) KRAS (HKe3-wtKRAS) and mtKRAS (HKe3-mtKRAS) were measured in 3DF culture with 11 BrUrd derivatives. Results: EdU was strongly incorporated into newly-synthesized DNA from HKe3-mtKRAS cells compared to HKe3-wtKRAS in 2D and 3DF culture. 3-Deaza-cytarabine, which has properties of BrUrd and cytidine, was the most effective inhibitor of HKe3-mtKRAS spheroids with the least toxicity to HKe3-wtKRAS. Growth suppression of 3-deaza-cytarabine was stronger than cytarabine in 2D culture, and toxicity was lower than gemcitabine in long-term 3DF culture. Conclusion: 3-Deaza-cytarabine exhibits properties useful for the treatment of CRC patients with mtKRAS.
- Colorectal cancer
- KRAS
- 3D floating culture
- nucleoside analogue
- 5-bromouridine (BrUrd)
- 4-Amino-1-(β-D-arabinofuranosyl)-2(1H)-pyridinone (3-deaza cytarabine)
Anticancer nucleosides include several analogues which fall into the categories of physiological pyrimidine and purine nucleosides. Pyrimidine analogues are further categorised into cytidine and uridine (or thymidine) analogues (1). Several pyrimidine analogues were synthesized and evaluated (2), and the first of these analogues to be approved by the US Food and Drug Administration (FDA) was the cytidine analogue, cytosine β-D-arabinofuranoside hydrochloride (cytarabine), that was approved in 1969 for the treatment of acute leukemia (3). In 1996, the FDA approved another cytidine analogue called 2’,2’-difluorodeoxycytidine (gemcitabine) (4). Gemcitabine has more favourable pharmacokinetic properties including greater solubility and lower toxicity to normal cells than cytarabine both in vitro and in vivo and has been used for the treatment of patients with solid tumours, including pancreatic cancers, lung cancers and colorectal cancers (CRCs) (5, 6). In these tumours a critically involved signalling pathway associated with oncogenic mutations in the Kirsten rat sarcoma viral oncogene homologue (KRAS) gene was identified (7). Furthermore, clinical studies have shown that patients with mutated (mt) KRAS show a worse response to gemcitabine-based therapy than those with wild-type (wt) KRAS (8). However, drugs selectively targeting oncogenic KRAS have not yet been clinically developed (9). Recently, we established a three-dimensional floating (3DF) culture system to preliminary screen chemical libraries which inhibit mtKRAS-mediated signaling (10). As a result, the uridine analogue, 5-bromouridine (BrUrd), was identified as a novel molecule that may be able to selectively target HKe3-mtKRAS spheroids and may exhibit low level toxicity to wtKRAS spheroids.
In order to determine the effects of 11 BrUrd derivatives in CRC with mtKRAS in vitro, 3DF culture was performed using the HKe3 cells overexpressing wtKRAS (HKe3-wtKRAS) and mtKRAS (HKe3-mtKRAS) (10). Our results suggest that 3-deaza-cytrabine may be an effective treatment for human colorectal cancer.
Materials and Methods
Reagents. BrUrd derivatives were kindly provided by RIKEN Natural Products Depository (NPDepo, Saitama, Japan). Chemical distances were determined by the Jaccard similarity index (11). Cytosine β-D-arabinofuranoside hydrochloride:cytarabine and gemcitabine hydrochloride were purchased from Sigma-Aldrich (St.Louis, MO, USA).
EdU incorporation assay. 4×104 HKe3-wtKRAS cells and 1×104 HKe3-mtKRAS cells, which were established in an earlier study (10), were seeded in LabTek 8-chamber slides (Nunc, Rochester, NY, USA) for 2D culture or in a 96-well plate with an ultra-low attachment surface and a round bottom (Corning Inc., Corning, NY, USA) for 3DF culture at 37°C for three days. Cells were treated with EdU for an additional 16 h. EdU was visualized using the Click-iT™ Plus EdU Imaging Kits Alexa (Life Technologies Corporation, Carlsbad, CA, USA) using the manufacturer's protocol.
Two-dimensional (2D) cell culture. HKe3-wtKRAS and HKe3-mtKRAS cells were cultured in Dulbecco's modified Eagle's medium (DMEM)-high glucose (Life Technologies Corporation) supplemented with 10% fetal bovine serum, 1% penicillin/streptomycin/Glutamine (Life Technologies Corporation), 600 μg/ml G418 (Wako, Osaka, Japan), and 2 μg/ml puromycin (Wako) as previously described (10, 12-19).
3D floating cell culture. HKe3-wtKRAS and HKe3-mtKRAS cells were seeded in 96-well plates with ultra-low attachment surfaces and round bottoms (Corning Inc.). Cells were cultured for 7 days in a CO2 incubator as previously described (10). Photomicrographs of cells were taken and analysed using an IN Cell Analyzer 1000 (GE Healthcare, Little Chalfont, UK) and IN Cell Developer Toolbox (GE Healthcare). The relative growth rate was calculated from a comparison of the area of control spheroids at day three.
Cell growth assay in 2D culture. 4×104 HKe3-wtKRAS cells and 1×104 HKe3-mtKRAS cells, which were established as previously described (10), were seeded in 96-well microplates (Corning) at 37°C for six days, and the absorbance of formazan solutions was measured using a 3-(4,5-dimethlthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay-based Cell Counting Kit-8 (Dojindo, Kumamoto, Japan) according to the supplier's instructions. The relative growth rate was calculated from a comparison of the absorbance obtained from cells treated with dimethyl sulfoxide (DMSO) alone as a vehicle control.
Statistical analyses. All experiments were performed in triplicate. Data are presented as means±standard deviations. Statistical analyses were performed using unpaired two-tailed Student's t-test in Microsoft Excel. p-Values of less than 0.05 were considered statistically significant.
Results
EdU incorporation in HKe3-wtKRAS and HKe3-mtKRAS cells grown in 2D and 3DF cultures. During the first screening, the uridine analogue, BrUrd, was identified as a candidate drug that inhibits the growth of HKe3-mtKRAS spheroids but not HKe3-wtKRAS spheroids (data not shown). A total of 11 BrUrd derivatives were selected from 4000 RIKEN libraries of natural products using chemical distance (11) (Table I). To address the hypothesis that BrUrd derivatives would be more strongly incorporated into HKe3-mtKRAS cells to suppress the growth of tumour cells than HKe3-wtKRAS cells in 2D and 3DF cultures, EdU was used, another uridine analogue, to visualize DNA incorporation. Cells were plated at day 0 and treated with EdU overnight at day 3. EdU staining was performed at day 4 (Figure 1A). EdU was more strongly incorporated into HKe3-mtKRAS cells than HKe3-wtKRAS cells in 2D (Figure 1B) and 3DF (Figure 1C) cultures under the same experimental conditions, suggesting BrUrd derivatives, including uridine analogues may be selectively incorporated into tumours with mtKRAS and suppress tumour growth.
BrUrd derivatives inhibit the growth of HKe3-mtKRAS spheroids. To examine the effects of BrUrd derivatives on cell proliferation, cells were treated with 11 BrUrd derivatives or DMSO alone in 3DF culture. To make the volume of HKe3-wtKRAS spheroids and HKe3-mtKRAS spheroids at day 3 uniform, 4×104 HKe3-wtKRAS cells and 1×104 HKe3-mtKRAS cells were plated and the area of spheroids was measured at days 3 and 7 (Figure 2A). The scoring method shown in Table II was also established. The area of HKe3-mtKRAS spheroids with 16.6 μM and 50.0 μM of BrUrd at day 7 were 0.81-fold (p=0.049) and 0.76-fold (p=0.0023) compared to control and were respectively smaller in comparison to those of the HKe3-wtKRAS spheroids treated with DMSO alone at day 7 (Figure 2B). The area of HKe3-mtKRAS spheroids with 16.6 μM and 50.0 μM of 3-deaza-cytarabine at day 7 were 0.76-fold (p=0.0038) and 0.56-fold (p=0.0009) smaller in comparison to that of HKe3-wtKRAS spheroids treated with DMSO alone at day 7, respectively (Figure 2B). These results suggest that 3-deaza-cytarabine suppresses spheroid growth more than BrUrd at the concentration of 16.6 μM. To test the late effect of these drugs from day 3 to day 7, the area of HKe3-wtKRAS and -mtKRAS spheroids with 16.6 μM and 50.0 μM of BrUrd derivatives at day 7 were compared to that at day 3 (Figure 2C). The area of HKe3-wtKRAS and HKe3-mtKRAS spheroids with DMSO at day 7, were 1.13-fold and 3.45-fold larger than the HKe3-wtKRAS spheroids at day 3, respectively (Figure 2B and C). Among the BrUrd derivatives showing significant (p<0.05) suppression of growth of HKe3-mtKRAS spheroids compared to those with DMSO control (3.45), the increased ratio with 16.6 μM of BrUrd, 5-Bromo-2’-deoxyuridine (BrdU), NPD7328, azidothymidine (AZT), 3-deaza cytarabine, 6-azauridine and NPD6666 from day 3 to day 7 was 1.10, 1.23, 1.89, 1.76, 1.20, 1.59, and 1.31-fold (Figure 2C), respectively, while the efficacy of 50.0 μM was 1.07, 1.27, 1.28, 1.42, 0.98, 1.45 and 1.19-fold greater (Figure 2C). These results suggest that BrUrd (1.07) and 3-deaza-cytarabine (0.98) at 50.0 μM concentration showed greater efficacy for growth suppression than the other candidates. On the other hand, the increased ratio of HKe3-wtKRAS spheroids with 16.6 μM and 50.0 μM of BrUrd, 5-Bromo-2’-deoxyuridine (BrdU), NPD7328, azidothymidine (AZT), 3-deaza cytarabine, 6-azauridine and NPD6666 from day 3 to day 7 was not significantly decreased compared to that with DMSO control. Notably, the increased ratio of HKe3-wtKRAS with 50.0 μM of 3-deaza-cytarabine was 1.14-fold larger than that with DMSO control (Figure 2C), suggestive of a low cytotoxicity of 3-deaza-cytarabine.
The efficacy of cytidine analogues for HKe3-wtKRAS and HKe3-mtKRAS cells grown in 2D culture. Structurally, 3-deaza-cytarabine is similar with both uridine analogues and cytidine analogues. To compare the efficacy of 3-deaza-cytarabine with other cytidine analogues which are currently being used in clinical settings, including cytarabine and gemcitabine (2), the effect of cytarabine, 3-deaza-cytarabine and gemcitabine in 2D culture was first examined. Cells were plated at day 0 and treated with drugs from day 0 to day 6 (Figure 3A). An increase in the concentration of cytarabine from 0.1 to 5.0 μM did not cause any statistically significant differences in cell proliferation of HKe3-wtKRAS and -mtKRAS cells in 2D culture (Figure 3B). While culturing cells with 3-deaza-cytarabine and gemcitabine show growth suppression of both HKe3-wtKRAS and - mtKRAS cells in 2D culture (Figure 3C and D). These results suggest that the growth suppression of 3-deaza cytarabine and gemcitabine is stronger than that of cytarabine in HKe3-wtKRAS and -mtKRAS cells in 2D culture. The 0.1 μM concentration of 3-deaza-cytarabine did not show any effect on cell proliferation (Figure 3C), however, the 0.1 μM of gemcitabine significantly decreased proliferation of both HKe3-wtKRAS and -mtKRAS cells in 2DC (Figure 3D), suggesting that the cytotoxicity of gemcitabine is higher than that of 3-deaza-cytarabine.
The long-term effect of BrdU, 3-deaza-cytarabine, and gemcitabine for HKe3-wtKRAS and-mtKRAS spheroids. To determine the cytotoxicity of pyrimidine (uridine and cytidine) analogues, including BrdU, 3-deaza-cytarabine and gemcitabine with low concentrations of drugs during long term exposure, a long-term 3DF culture was performed. Cells were plated at day 0 and treated with drugs in serial dilutions and the area of spheroids was measured every three to four days until day 27 (Figure 4A). The area of HKe3-wtKRAS spheroids with DMSO alone at day 27 was 2.48-fold larger when compared to that at day 3 (Figure 4B). On the other hand, the area of HKe3-mtKRAS spheroids with DMSO alone at day 27 were 15.75-fold larger when compared to that at day 3 (Figure 4B). Treatment of HKe3-mtKRAS spheroids by serially-diluted 3-deaza-cytarabine showed that the concentration required to achieve a 50% of the maximal inhibition in cell proliferation is approximately 0.5 μM (Figure 4B). The area of HKe3-wtKRAS spheroids with BrdU and 3-deaza-cytarabine at day 17 is similar with DMSO control (Figure 4C), suggesting that both drugs do not display cytotoxicity to cells with wtKRAS. On the other hand, cytotoxicity of gemcitabine was observed in both HKe3-wtKRAS and -mtKRAS spheroids at a concentration of 0.1 μM. Furthermore, the area of HKe3-mtKRAS spheroids with 0.1 and 0.5 μM of BrdU was larger than that of 3-deaza-cytarabine at day 17 (Figure 4C), suggesting that the cytotoxicity of 3-deaza-cytarabine is stronger than that of BrdU.
Discussion
In this study, it was demonstrated for the first time that BrUrd derivatives selectively reduce the growth of CRC spheroids with mtKRAS. In particular, the structure of 3-deaza-cytarabine is similar to both BrUrd and cytarabine (Table I). The uracil of BrUrd and the cytosine of cytarabine are similar in structure and both BrUrd and 3-deaza-cytarabine have arabinose as the sugar component which is thought to be the part that is incorporated into the DNA. Because 3-deaza-cytarabine lacks a nitrogen at the position 3 of the cytosine of cytarabine, 3-deaza-cytarabine correctly belongs to 3-deaza-cytidine analogue. Since, 3-deaza uridine has been demonstrated to have significant antitumor activity (20), 3-deaza-cytarabine may also possess strong antitumor properties.
BrUrd, EdU and BrdU have been reported to be used for measuring DNA incorporation (21, 22). Recent work also suggested that these uridine analogues may play a role in the strong suppression of cancer cell proliferation (23-25). Notably, the uridine analogue, EdU was more strongly incorporated into newly synthesized DNA from Hke3-mtKRAS cells than from HKe3-wtKRAS cells in 2D and 3DF cultures (Figure 1). From the view of structural similarities between BrUrd and 3-deaza-cytarabine (Table I), both analogues may selectively suppress the growth of mtKRAS tumours via the acceleration of DNA incorporation.
3-Deaza-cytarabine belongs to a set of cytidine analogues which include cytarabine and gemcitabine. Growth suppression by 3-deaza-cytarabine is stronger than cytarabine in 2D culture (Figure 3B) and its cytotoxicity is lower than gemcitabine in long-term 3DF culture (Figure 4C). Because the growth of HKe3-mtKRAS spheroids represent in vivo CRC growth and HKe3-wtKRAS spheroids represent the growth of normal colonic crypts in vivo (14), the selective suppression of 3-deaza-cytarabine for HKe3-mtKRAS spheroids indicates the utility of 3-deaza-cytarabine as an anticancer agent with low toxicity for tumours with mtKRAS. Furthermore, the suppressive effect of cytarabine is reported to be very weak for solid tumors (26) and the clinical use of gemcitabine is often limited due to primary/acquired drug resistance (27). Therefore, there is still room to further develop/improve novel nucleoside analogues used for the treatment of cancer patients with mtKRAS.
Using our culture system and scoring method (Table II), other compounds have already been identified in our lab showing selective effects for tumours with mtKRAS in 3D microenvironments and having different structures to BrUrd (data not shown).
Acknowledgements
The Authors thank Takami Danno, Yuriko Isoyama and Yumiko Hirose for their technical assistance. This study was supported by the Ministry of Education, Culture, Sports, Science and Technology of Japan.
Footnotes
↵* These Authors contributed equally to this study.
- Received April 11, 2018.
- Revision received May 22, 2018.
- Accepted May 23, 2018.
- Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved