Abstract
Background: Resistance to chemotherapy is a main problem in cancer. The search for new effective compounds that can increase sensitivity of resistant cells to existing chemotherapeutics is an urgent need. In previous studies, it has been demonstrated that steroid derivatives showed promising results concerning their capacity to modulate resistance of multidrug-resistant cell lines. Materials and Methods: Steroid derivatives were studied for their growth-inhibitory effect, cytotoxicity, reversal of multidrug resistance, apoptosis induction, and interaction with doxorubicin on multidrug resistant human ATP-binding cassette, sub-family B, member 1 (ABCB1) gene-transfected mouse T-lymphoma cell line, and human PC-3 and LNCaP prostate cancer cell lines in vitro. The steroidal interaction with P-glycoprotein (ABCB1) was investigated by molecular docking. Results: Both the activity of steroid derivatives on inhibition of the ABCB1 pump and their interaction with doxorubicin are dependent on the substituent groups of the investigated steroidal structures. Even though the investigated steroid derivatives were found to have limited antiproliferative effect on the three different cancer cell lines, in combination with doxorubicin, most of them acted as good potentiators. The binding energies from molecular docking ranged from −6.43 to −9.88 kcal/mol. The predicted inhibition constants ranged from 0.1 to 10.1 μM. A significant negative correlation was found between binding energy and fluorescence activity ratio (R=−0.5, p=0.015). Conclusion: The effective compounds can be candidates of model molecules for possible application in the treatment of multidrug resistant cancer in rational drug design.
Prostate cancer has become the most common malignant disease among men, and the second leading cause of male cancer deaths in the USA. In the EU, prostate cancer is the most common cancer in men and over 417,000 new cases are diagnosed every year (1). Mortality from prostate cancer is increasing disproportionately with the aging of the male population within an overall population growth, and the explanation for this alarming trend is still elusive (2). In Hungary, for example, approximately 1250 men die from prostate cancer each year (3).
It is known that testosterone and 5α-dihydrotestosterone (5α-DHT) are androgens required for the development of both normal prostate and prostate cancer. Prostate-specific antigen (PSA), also known as gamma-seminoprotein or kallikrein-3 (KLK3), is a glycoprotein enzyme encoded in humans by the KLK3 gene. PSA is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland (4). PSA is present in small quantities in the serum of men with healthy prostates, but is often elevated in the presence of prostate cancer or other prostate disorders (4). Since the PSA test is used for prostate cancer diagnosis, prostate cancer is detected at more localized/early stages. Nevertheless, depending on the tumor differentiation stage, there is recurrence of the disease in 10-40% of cases, which require further treatment. Once hormonal therapy fails and disease progress to a castration-resistant stage, the question of chemotherapy arises (5).
Multidrug resistance plays a crucial role in the failure of treatment of all kinds of cancer, including prostate cancer. For the past 40 years, researchers have been investigating the various mechanisms by which cancer cells grown in tissue culture become resistant to anticancer drugs (6). These mechanisms include loss of a cell surface receptor acting as a transporter for a drug to the inside of the cell, increased metabolism of a drug and its detoxification, alteration by mutation of its specific target(s) (7), and the elevated expression of ATP-dependent drug-efflux pumps, therefore reducing the accumulation of anticancer agents inside the cell, among others (8).
The efficient synthesis of substituted androstane by A-ring modification of picolinyldieneandrostane derivatives has been reported (9). It has been suggested that this compound specifically interacts with cytochrome P450 17α-hydroxylase/17,20-lyase (CYP17) with similar affinity to the abiraterone type of androstane drug clinically used in the treatment of prostate cancer. It has been found that different (17E)-picolinilidene-androstanes have selective antiproliferative activity against PC-3 prostate cancer cells, suggesting that substituted steroidal compounds containing amino and amido groups can be exploited as lead compounds in the development of new anticancer compounds for the treatment of prostate cancer (9, 10). Testosterone, progesterone and other important natural steroids contain ketone groups in position 3. Reduction of these groups into 3β-hydroxy causes the loss of hormonal activity. In the case of estrogens, alkylation of the 3-phenolic hydroxyl group results in similar disappearance of their activity, therefore we also prepared derivatives of 3β-hydroxy-pregnenes, -androstenes and 3-alkoxy-estratrienes. Amide groups were chosen as substituents. We considered that amide groups were able to attach to the peptide groups of proteins in cell membranes by hydrogen bridges, initiating an effect on the cell membrane components, including the ATP-binding cassette, sub-family B, member 1 (ABCB1) transporter. The starting steroids were transformed into 3-, 16-, and 21- amino derivatives and were acylated first with N- protected natural L amino acids. More simple compounds were also prepared in which the amide group is not a peptide-like moiety, but a simple acyl amide, produced by acetylation of an alkyl-amino group (compounds 13, 16-19, and 20, Figure 1).
In the present study, substituted steroids were synthesized by using amido substituents and tested for their in vitro activity, including antiproliferative and cytotoxic effect, their capacity to inhibit the function of the ABCB1 membrane transporter, apoptosis induction effect and activity in combination with known chemotherapeutic agents, such as doxorubicin.
Materials and Methods
Compounds. Aminosteroids 14 and 15 were prepared from oximes of the corresponding ketosteroids by sodium tetrahydroborate reduction in the presence of NiCl3, as described previously (11). The obtained aminosteroids were acylated with N-terc-butil-oxy-carbonyl (BOC)-protected amino acids by mixed anhydride method (1-5) and the protecting group was eliminated with dry hydrogen chloride in dioxane solution, affording amine hydrochlorides 6-12. Alkyl-aminomethylene steroids were prepared from 16-hydroxyme thylene-17-ketosteroids and primary amines and acetylated with acetic anhydride (compounds 16-20) (12, 13). The D-condensed heterocyclic steroid 21 was prepared from 16-hydroxymethylene-3-methoxy-estra-1,3,5(10)trien-17-one with guanidine (14, 15). The 16-aminomethyl-androstene derivative 13 was synthesized from the corresponding 16-methylene-17-ketone by addition of n-propylamine and selective O,O-desacetylation of the full acetylated product (15). The chemical structures of the steroid derivatives evaluated in this study are shown in Figure 1. Samples of the above mentioned compounds were dissolved in dimethyl sulfoxide (DMSO) as 2.0 mg/ml stock solutions. Verapamil as positive control, DMSO and N-BOC-isoleucine-O-pentachloro-phenylester (compound 23) were obtained from Sigma-Aldrich, St. Louis, MO, USA.
Cell cultures. The parental L5178 mouse T-cell lymphoma cells (L5178 PAR) (ECACC cat. no. 87111908, obtained from the Food and Drug Administration, Silver Spring, MD, USA) were trans fected with pHa ABCB1/A retrovirus, as previously described by Cornwell et al. (16). The multidrug- resistant (MDR) ABCB1-expressing cell line L5178Y was selected by culturing the trans fected cells in medium containing 60 ng/ml colchicine. L5178 PAR mouse T-cell lymphoma cells and the MDR L5178Y human ABCB1-transfected subline were cultured in McCoy's 5A medium supplemented with 10% heat inactivated horse serum, 200 mM L-glutamine, and penicillin-streptomycin mixture at 100 U/l and 10 mg/l, respectively.
The human prostate cancer cell lines PC-3 (ATCC® CRL-1435) and LNCaP (ATCC® Cat. No. CRL-1740) were purchased from LGC Standards GmbH, Wesel, Germany. The cells were grown in RPMI-1640 medium with 2 mM L-glutamine and 10% fetal bovine serum supplemented with antibiotics. Adherent human cancer cells were detached with 0.25% trypsin and 0.02% EDTA for 5 min. The cell lines were incubated in a humidified atmosphere (5% CO2, 95% air) at 37°C. The LNCaP cell line possesses androgen and oestrogen receptors, while the PC-3 cell line does not contain either of these receptors and is a suitable model for the hormone-resistant state.
Assay for antiproliferative effect of the compounds. The effects of increasing concentrations of compounds on cell growth were determined in 96-well flat-bottomed microtiter plates. The compounds were serially diluted in 100 μl of McCoy's 5A or RPMI-1640 medium, respectively. A total of 6×103 mouse T-cell lymphoma cells (PAR or MDR) or 1×104 PC-3 prostate cancer cells in 100 μl of medium were then added to each well, with the exception of the medium control wells. The culture plates were further incubated at 37°C under 5% CO2 for 72 h. At the end of the incubation period, 20 μl of 3-[4.5-dimethylthiazol-2-yl]-2.5 diphenyltetrazolium bromide (MTT) (Sigma, St. Louis, MO. USA) solution (from a 5 mg/ml stock) were added to each well and after a further 4 h, 100 μl of 10% sodium dodecyl sulfate (Sigma) in 0.01 N HCl were measured into each well. The culture plates were further incubated at 37°C overnight. The cell growth was determined by measuring the optical density (OD) at 550 nm (ref. 630 nm) with a Multiscan EX ELISA reader (Thermo Labsystem, Cheshire, WA, USA). In the assay, the solvent did not have any effect on the cell growth at the concentrations used for half maximal inhibitory concentration (IC50) calculations. IC50 values and the standard error of the mean (SEM) of triplicate experiments were calculated using GraphPad Prism software version 5.00 for Windows with nonlinear regression curve fit (GraphPad Software, San Diego, CA, USA; www.graphpad.com).
Assay for reversal of MDR in mouse T-lymphoma cells. The cell density of L5178 MDR and L5178Y PAR cell lines was adjusted to 2×106 cells/ml, and they were re-suspended in serum-free McCoy's 5A medium and distributed in 0.5 ml aliquots into Eppendorf centrifuge tubes. The tested compounds were added at a final concentration of 2 μg/ml and the samples were incubated for 10 minutes at room temperature. Verapamil (Sigma) was applied as positive control (16) at 10 μg/ml. DMSO was added to the negative control tubes at the same volume used for the tested compounds. No activity of DMSO was observed. Next, 10 μl (5.2 μM final concentration) of the fluorochrome and ABCB1 substrate rhodamine 123 (Sigma) was added to the samples and the cells were incubated for a further 20 minutes at 37°C, then washed twice and re-suspended in 0.5 ml phosphate buffer saline (PBS) for analysis. The fluorescence of the cell population was measured with a Partec CyFlow® flow cytometer (Partec, Münster, Germany). The percentage mean fluorescence intensity was calculated for the treated MDR cells as compared with the untreated cells. A fluorescence activity ratio (FAR) was calculated based on the following equation (16) on the basis of the measured fluorescence values:
A checkerboard microplate method was applied to study the effect of drug interactions between the steroidal derivatives and chemotherapeutic drug doxorubicin on PC-3 cancer cells. The dilutions of doxorubicin were made in a horizontal direction in 100 μl, and the dilutions of the compounds vertically in the microtiter plate in 50 μl. The cells were re-suspended in culture medium and distributed into each well in 50 μl containing 6×103 cells. The plates were incubated for 72 h at 37°C in a CO2 incubator. The cell growth rate was determined after MTT staining, as described above. Combination index (CI) values at 50% growth inhibition (ED50) were determined using CompuSyn software (http://www.combosyn.com; ComboSyn, Inc., Paramus, NJ, USA) to plot four to five data points for each ratio. CI values were calculated by means of the median-effect equation (17), where CI <1, CI=1, and CI >1 represent synergism, additive effect (or no interaction), and antagonism, respectively.
Apoptosis assay. The capacity of the compounds to induce apoptosis was investigated using human PC-3 prostate cancer cell line. The cell density was adjusted to 5×105 cells/ml and cells were distributed in 0.5 ml aliquots into 24-well plates. The apoptosis inducer 12H-benzo[α]phenothiazine (M627) was kindly provided Professor Noborul Motohashi (Meiji Pharmaceutical University, Tokyo, Japan) used as positive control at 25 μg/ml. Compounds were tested at 4 μg/ml. Wells containing no M627 or steroidal compounds served as negative controls. The cells were incubated at 37°C for 3 h and at the end of the incubation period, the culture medium was removed, and the cells were washed with PBS and 0.5 ml of fresh culture medium was added to the cells. Samples were then transferred to 24-well culture plates and further incubated overnight at 37°C under 5% CO2. Apoptotic activity of the compounds was evaluated using Annexin-V FITC Apoptosis Detection Kit (Cat. No. PF 032; Calbiochem, Merck KGaA, Darmstadt, Germany) according to the manufacturer's instructions. The fluorescence of cell population was analyzed immediately using a Partec CyFlow® flow cytometer (Partec).
Molecular docking. The 2D structures of the chemical entities were drawn and later energy-minimized into 3D structures using Corina Online Demo (18). All 3D structures were saved in PDB format ready to be docked. Molecular docking was conducted following a protocol previously reported by us (19). In brief, the X-ray crystallography-based structure of mouse P-glycoprotein and the homology-modelled structure of human P-glycoprotein were set as the rigid receptor molecule. The X-ray crystallography protein-based structures were first processed with AutodockTools-1.5.6rc3 (20) by the addition of essential hydrogen atoms to overcome the problem of incomplete structures due to missing atoms or water molecules. The output file after preparation was in PDBQT format, where information about atomic partial charges, torsion degrees of freedom and different atom types were added, e.g. aliphatic and aromatic carbon atoms or polar atoms forming hydrogen bonds. A grid box was then constructed to define docking spaces. The dimensions of the grid box were set around the whole P-glycoprotein molecule such that the ligand was able to freely move and rotate in the docking space. The grid box consisted of 126 grid points in all three dimensions (X, Y and Z) each separated by a distance of 1 Å. Energies at each grid point were then evaluated for each type of atom present in the ligand, and the values were then used to predict the energy of a particular ligand configuration. Docking parameters were set to 250 runs and 2,500,000 energy evaluations for each cycle. Docking was performed by Autodock4 (20) using the Lamarckian algorithm. The corresponding binding energies and the number of conformations in each cluster were attained from the docking log files. The binding energies of the 23 compounds were correlated with their FARs using Pearson correlation coefficient.
Results
Twenty-three derivatives were synthesized and their antiproliferative (Table I) and cytotoxicity activities were studied on PAR, MDR, LNCaP, and PC-3 cell lines. Evaluation of the antiproliferative activity of the compounds revealed that compounds 8 and 10-14 were the most active against the parental mouse T-lymphoma cell line, with IC50 values of less than 5 μg/ml. With the exception of compounds 9 and 13, all the above-mentioned compounds and compound 22 were also active against the MDR cell line. With respect to the two different prostate cancer cell lines, the most effective compounds were 3, 12, 18, 19, and 23 against LNCaP cells, and compound 12 against PC-3 cells. Therefore, as per the results above, compound 12 was the only one with strong activity against all four cell lines studied.
The capacity of the compounds to inhibit the ABCB1 transporter was studied on MDR mouse T-lymphoma cells that were transfected with the human ABCB1 gene (Table II). The MDR-reversal effect was studied at 2 μg/ml. Aminosteroid compounds acylated with BOC amino acids 1-5 and 9 and even simple N-acetyl derivatives 13, 16, 18, 19 presented remarkable activity in the reversal of MDR of the MDR mouse T-lymphoma cell line, with FAR values of between 29 and 77. Steroids containing free amino groups or amine-hydrochloride substituents showed weaker or no activity on MDR reversal as measured by accumulation of rhodamine-123. Interestingly, compound 12 presented only mild inhibition at the concentration used. Concerning the activity of compounds expressed in μM, the most active compound was compound 18 because at a concentration of 4.4 μM, the FAR of compound 18 was 77.41. Moreover, compounds 1, 3, 13, 16, and 19 were also highly active.
The apoptosis-inducing effect of the compounds was measured at the non-toxic concentration of 4 μg/ml on the PC-3 cell line (data not shown). It was observed that there was no relevant activity of these compounds on apoptosis induction: the proportion of early apoptosis was between 1-6%, late apoptosis and necrosis between 1-5%, and cell death was around 1%. There was no significant difference when comparing these data with those of the untreated control.
The activity of the steroidal compounds in combination with doxorubicin was analyzed using checkerboard assay for PC-3 cells (Table III). It was observed that the effect of these compounds on the activity of doxorubicin ranged from ineffective to strongly synergistic. Strongly synergistic effects of modified steroids with doxorubicin were produced in the case of five compounds, namely 4, 9, 10, 14, and 22. Synergy was found with derivatives 1, 2, 8, 12, 16, 17, 19, and 23. Two compounds, 3 and 18, were ineffective in this study. The IC50 of compounds 5-7, 11, 13, 15, 20 and 21 could not be calculated and this fact did not allow the determination of the degree of interaction of these compounds with doxorubicin.
We investigated in silico the interaction of the panel of steroid compounds with P-glycoprotein, using verapamil as a positive control. All 23 compounds had binding energies lower than that of verapamil (Table IV). The binding energies ranged from −6.43 to −9.88 kcal/mol. The predicted inhibition constants ranged from 0.1 to 10.1 μM. The inter action of four of the test compounds, the control compound verapamil and the amino acids involved are shown in Figure 2. The binding sites of the compounds were found to coincide with the binding site of verapamil. The binding energies of the 23 compounds were correlated with their FARs using Pearson correlation coefficient (Figure 3). A significant negative correlation was found between the binding energies of the 23 compounds and their FARs (R=−0.5, p=0.015).
Discussion
The MDR transporters are one of the main causes of cancer treatment failures (21). Modulation of efflux pump-related mechanisms, in order to increase the activity of existing chemotherapeutics to which cancer becomes resistant, is one of the possible ways to overcome resistance. In our investigation, modified steroid derivatives without hormonal activity were chosen to achieve this effect.
Based on our studies, compounds 8, 10-12, 14, and 22 had effective antiproliferative activity against MDR mouse T-lymphoma cells. These compounds are aminoacylamide salts applied to three different steroid skeletons (androstane, androstene, and preganadienes). Compound 22 is an estrone ether derivative and it has a bidentate α-hydroxymethyleneketone arrangement on the D-ring, rendering the possibility of a 1,4-hydrogen donor-acceptor connection. On the other hand, compounds 3, 12, 18, 19, and 23 proved to be effective in the antiproliferative assays against the LNCaP prostate cell line.
In the MDR-reversal studies, the majority of the investigated compounds were effective: 1-5, 9, 13, 16-20 (Table II). Compounds 6, 8, 10-12, 14, 15, and 21 had only a weak effect. These latter molecules either contain amine hydrochloride, or have special structures; the latter three compounds (14, 15, and 21) are estrone ether derivatives. Aminosteroids with basical primary amino groups had practically no effect. The compounds of remarkable activity contain four different steroid skeletons, four different amino acyl parts (glycyl, alanyl, phenylalanyl and prolyl) and, in the case of the simpler molecules, three different alkyl (propyl, benzyl and acethoxyethyl) moiety. The only common structural element amongst them is the amide part, deriving from an α-amino acid or acetic acid. In compounds 18 and 19, a double bound is present in the A and B rings, in addition to there being no H atom at the C-5 position. This H atom either induces or is responsible for conformational changes in the four condensed rings with chair-like structure. In the case of compound 13, the hydrophobic steroid containing acyl-amide can help binding to the beta-turn of the ABCB1 protein. We suppose that the biologically active N-protected BOC compounds bind to particular beta-turns of the ABCB1 transporter. Possibly, there are preferred amino acids in the beta-turns that may have a key role in maintaining the functionally active conformations of the ABCB1 transporter localized in the cell membrane. Molecular docking is an effective computational technique which estimates the binding energy of a compound to the target protein. A scoring system is used to detect the ideal docking configuration. The amino acids involved in hydrophobic and hydrogen bond interactions are also predicted by the algorithm. Our results from molecular docking suggest that the compounds tested inhibit P-glycoprotein activity through binding to the drug-binding pocket, which is the same binding site as for verapamil. Most of the compounds with high activity, and verapamil, share one or more amino acids in their binding sites. It appears that there is a significant correlation between the binding energies of the compounds and their FARs. It can be suggested that molecular docking might be used as a tool predictive of the activity of steroidal compounds as inhibitors of P-glycoprotein.
A large number of compounds were able to enhance the activity of doxorubicin against the PC-3 cell line in combination experiments. Based on our results, compound 9 could be a lead compound for further investigations related to combined chemotherapy; in addition, compound 12 might be a promising candidate as an effective antiproliferative chemotherapeutic agent.
Acknowledgements
The study was supported by Szeged Foundation for Cancer Research, by the project TAMOP-4.2.2A-11/1/KONV-2012-0035, and by the Fundação para a Ciência e a Tecnologia (FCT), Portugal (PEsT-OE/SAU/UI0074/2011 and PEsT-OE/SAU/UI0074/2014). Ana Martins acknowledges the grant SFRH/BPD/81118/2011 provided by the FCT, Portugal. The Authors are grateful to the German Academic Exchange Service (DAAD) for a stipend to Sami Hamdoun. We are grateful to Mrs. Anikó Vigyikán Váradi for the preparation of the tissue cultures and technical assistance, and to Imre Ocsovszki for the flow cytometric measurements. They are also grateful to Kristóf Boa for the language editing of the manuscript.
- Received December 26, 2014.
- Revision received January 19, 2015.
- Accepted January 21, 2015.
- Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved