Abstract
Aim: To identify conditions that induce an increase in the sensitivity of highly Halaven (HAL)-resistant cancer cells compared to sensitive cells. Materials and Methods: We observed that drug-resistant KBV20C cells are highly resistant to HAL compared to other antimitotic drugs. The concentration required to treat KBV20C cells was almost 500-fold higher than that used to treat sensitive parent KB cells. In order to increase sensitization, HAL-treated KBV20C cells were co-treated with the repositioned drug, fluphenazine (FLU). Results: HAL and FLU co-treatment inhibited the growth and increased apoptosis via G2 arrest in HAL-treated KBV20C cancer cells. Sensitization by HAL-FLU affected retinoblastoma protein (pRB), pHistone H3 and pH2AX protein levels. FLU could also inhibit p-glycoprotein (P-gp) activity in HA-resistant KBV20C cells. These observations suggest that the mechanisms underlying FLU-HAL sensitization in resistant KBV20C cells involve both apoptosis and P-gp inhibition. Furthermore, both thioridazine (THIO) and mefloquine (MEF), but not azathioprine (AZA), sensitized HAL-treated KBV20C cells. Conclusion: These findings provide important information regarding the sensitization of HAL-resistant cells and indicate that FLU, THIO and MEF may have similar sensitization effects in highly HAL-resistant cells.
Antimitotic drugs, which target different binding sites on tubulin, are widely used for treating different cancers (1, 2). These compounds inhibit mitosis by targeting microtubules and preventing their polymerization or depolymerization (1-4). Halaven (HAL), also called eribulin, was recently developed and used in the clinic to treat resistant or metastatic cancer. HAL has been developed to overcome the resistance of cancer cells to routinely used antimitotic drugs. HAL targets the depolymerization of microtubules (5, 6). HAL is considered a promising drug for triple-negative breast cancer or certain resistant cancers (7, 8). Since patients are expected to develop resistance to HAL, identifying the mechanism(s) that underlie cell sensitization to HAL would be an important step in the development of more effective treatments by designing approaches to increase HAL-associated apoptosis.
In the present study, we determined that P-glycoprotein (P-gp)-overexpressing KBV20C cells are highly resistant to HAL compared to their resistance to other antimitotic drugs. Fluphenazine (FLU) was used as a neuroleptic or anti-emetic agent (9, 10). However, FLU has been recently shown to inhibit P-gp and is considered an anti-cancer chemopreventive agent (11, 12). We then tested whether co-treatment using HAL and FLU increases the sensitivity of highly HAL-resistant KBV20C cells. We further investigated the mechanism underlying HAL-FLU sensitization using various apoptotic assays and molecular signatures to determine the factors involved in the effects observed in HAL-resistant cancer cells co-treated with HAL and FLU. Additionally, we tested whether co-treatment with thioridazine (THIO), azathioprine (AZA) or mefloquine (MEF) sensitized HAL-treated KBV20C cells. Since these drugs have already been approved for the treatment of humans (13), they, as repositioned drugs, could be readily available for clinical use once their anti-cancer activities are better understood. These results will contribute to the development of FLU-, THIO- and MEF-based therapy for co-treatment of cancer in highly HAL-resistant patients.
Materials and Methods
Reagents. FLU, THIO, MEF and verapamil (VER) were purchased from Sigma-Aldrich (St.Louis, MO, USA). AZA was purchased from Selleckchem (Houston, TX, USA). Vinblastine (VIB) was purchased from Enzo Life Sciences (Farmingdale, NY, USA). Aqueous solutions of HAL (Eisai Korea, Seoul, South Korea) were obtained from the National Cancer Center in South Korea.
Antibodies. Antibodies against pGSK3β, pHistone H3, proliferating cell nuclear antigen (PCNA) and cleaved-poly(ADP-ribose) polymerase (C-PARP) were from Cell Signaling Technology (Danvers, MA, USA). Antibodies against glyceraldehyde 3-phosphate dehydrogenase (GAPDH), survivin and retinoblastoma protein (pRb) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibody against pH2AX was from Abcam (Cambridge, UK).
Cell culturing. Human oral squamous carcinoma cell lines, KB and its multidrug-resistant subline, KBV20C, were obtained from Dr. Yong Kee Kim (College of Pharmacy, Sookmyung Women's University, Seoul, Republic of Korea) and have been previously described (14-16). All cell lines were cultured in RPMI 1640 containing 10% fetal bovine serum, 100 U/ml penicillin and 100 μg/ml streptomycin (WelGENE, Daegu, South Korea).
Microscopic observation. Cells grown in 6-well plates were treated with the indicated drugs for the indicated times. The medium was removed and PBS was added in each dish. Cells were examined in two independent experiments immediately using an Axio observer.Z1 fluorescence inverted microscope (Carl Zeiss, Oberkochen, Germany) with a 5× or 10× objective lens (Carl Zeiss EC Plan-Neofluar).
Fluorescence-activated cell sorting (FACS) analysis. FACS analysis was performed as previously described (17-19). Cells were grown in 60-mm diameter dishes and treated with the indicated drugs for the prescribed times. The cells were then dislodged by trypsin and pelleted by centrifugation. The pelleted cells were washed thoroughly with PBS, suspended in 75% ethanol for at least 1 h at 4°C, washed with PBS and re-suspended in a cold propidium iodide (PI) staining solution (100 μg/ml RNase A and 50 μg/ml PI in PBS) for 30 min at 37°C. The stained cells were analyzed in two independent experiments for relative DNA content using a FACSCalibur flow cytometry system (BD Bioscience, Franklin Lakes, NJ, USA).
Annexin V analysis. Annexin V analysis was conducted using the annexin V-fluorescein isothiocyanate (FITC) staining kit (BD Bioscience) as previously described (17-19). Cells were grown in 60-mm diameter dishes and treated with the indicated drugs for the prescribed times. The cells were then dislodged by trypsin and pelleted by centrifugation. The pelleted cells were washed with PBS. Cells in 100 μl of binding buffer received 5 μl of Annexin V-FITC and 5 μl of PI and were, then, incubated for 15 min at room temperature. The stained cells were analyzed using a FACSCalibur flow cytometry system (BD Bioscience). Two independent experiments were performed.
Rhodamine and calcein-AM uptake tests. These tests were used for determination of ability for inhibition of P-gp using a previously described method (17-19). Briefly, cells grown in 6-well plates were treated with indicated drugs and incubated for 24 h at 37°C. Cells were then incubated with and 1 μg/ml rhodamine or 0.1 μg/ml calcein-AM for 1 h 30 min at 37°C. The medium was removed and the cells were washed with PBS. The stained cells were analyzed in two independent experiments using a FACSCalibur flow cytometry system (BD Bioscience).
Western blot analysis. Total cellular proteins were extracted using a previously described trichloroacetic acid (TCA) method (17-19). Briefly, cells grown in 60-mm dishes were washed three times with 5 ml PBS. Next, 500 μl of 20% trichloroacetic acid (TCA) was added to each plate. The cells were then dislodged by scraping and transferred to Eppendorf tubes. Proteins were pelleted by centrifugation for 5 min at 3,000 rpm and re-suspended in 1M Tris-HCl (pH 8.0) buffer. The total protein concentrations were estimated. The proteins were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and subjected to Western blot analysis as previously described (17-19).
Results
KBV20C cells, highly resistant to HAL, are sensitized by FLU co-treatment. KBV20C cells present an antimitotic drug-resistant phenotype via P-gp overexpression (14-16). When testing the degree of resistance of KBV20C cells to HAL, we determined that KBV20C cells are highly resistance to HAL. In fact, in KBV20C cells, the necessary concentration of HAL required to observe a similar phenotype was approximately 500-fold higher than that used in parent drug-sensitive KB cells (Figure 1A). These data suggest a 500-fold difference in HAL half-maximal inhibitory concentration (IC50) between sensitive KB and resistant KBV20C cells. Compared to previous studies assessing the IC50 of KB and KBV20C cells using other antimitotic drugs, such as vinblastine, vincristine, paclitaxcel, docetaxcel and colchicines (1-4), KBV20C cells are highly resistant to HAL. Verapamil co-treatment with HAL overcame KBV20C resistance (data not shown or see Figure 3C and D) confirming that HAL-resistance in KBV20C cells involves the pumping-out ability of the cells associated with P-gp overexpression. It also suggests that HAL is a highly selective substrate pumped-out by P-gp in KBV20C cells. Therefore, we assumed that identifying the sensitization mechanisms of KBV20C cells can provide important information to overcome HAL-resistant phenotypes in the clinic.
FLU is a potential anti-cancer drug and a potential inhibitor of cancer-related Akt pathways (20). First, we tested whether FLU was a substrate for P-gp in drug-resistant KBV20C cells. As shown in Figure 1B, using various concentrations of FLU, we determined that KB and KBV20C presented a similar sensitization level, suggesting that FLU is not a substrate of P-gp in drug-resistant KBV20C cells. It also suggests that FLU can be used as an anti-cancer drug in resistant cells, including HAL-resistant cancer cells. Furthermore, we tested whether co-treatment with FLU could sensitize HAL-treated KBV20C cells. As shown in Figure 1C and D, HAL-FLU co-treatment reduced cellular proliferation and increased G2 arrest when compared to treatment with HAL or FLU alone. Collectively, our data show that KBV20C cells are highly resistant to HAL. HAL-FLU co-treatment resulted in reduced proliferation and increased G2 arrest. In addition, FLU single treatment sensitized drug-resistant KBV20C cells as much as KB cells with a similar IC50.
HAL-FLU sensitization of KBV20C involves both apoptosis and P-gp inhibition. We also confirmed that HAL-FLU co-treatment increased apoptosis, which was determined by annexin V staining (Figure 1E). A more detailed analysis of annexin V showed that higher HAL concentrations (30 μM HAL vs. 50 μM HAL) increased apoptosis when combined with FLU (Figure 2A), whereas single treatment using both 30 μM HAL and 50 μM HAL induced similar levels of apoptosis (Figure 2A). Thus, FLU can dose-dependently recover the anti-cancer ability of HAL.
Especially, the early stage of apoptosis was increased after HAL-FLU co-treatment, suggesting that increased G2 arrest finally resulted in the induction of apoptotic events. Moreover, HAL-FLU co-treatment increased C-PARP production (Figure 2B). When we assessed the levels of important proliferation-related protein or phosphorylation levels (21, 22), HAL-FLU co-treatment increased pRb, pHistone H3 and pH2AX (Figure 2B), thus suggesting that HAL-FLU co-treatment sensitizes KBV20C cells by reducing proliferation and increasing DNA damaging processes. Collectively, microscopic, FACS, annexin V and western-blot analyses indicated that FLU increased DNA damage and G2 arrest to increase apoptosis in HAL-treated resistant KBV20C cells.
We also assessed whether FLU inhibited P-gp. Our results showed that FLU inhibits P-gp activity (Figure 2C). However, P-gp inhibition by FLU was lower than that by a well-known P-gp inhibitor, verapamil (Figure 2C). Thus, FLU can be consider as a weaker P-gp inhibitor. FLU single treatment sensitized both resistant KBV20C and sensitive KB cells (Figure 1B). Thus, HAL-FLU sensitization in KBV20C cells involves both FLU cytotoxic and P-gp inhibitory effects.
FLU-HAL, THIO-HAL and MEF-THIO have similar sensitization effects in highly HAL-resistant KBV20C cells. We previously showed that THIO and MEF can sensitize KBV20C cells by co-treatment with antimitotic drugs (14, 19, 23). MEF can sensitize HAL-treated KBV20C cells to other types of antimitotic drugs, such as vinblastine, vinorelbine, paclitaxel or docetaxel (14). Here, we tested whether the combination of MEF or THIO with HAL would be more effective than FLU co-treatment. As shown in Figure 3A and B, C-PARP production and microscopic observations indicated that HAL-MEF, HAL-THIO and HAL-FLU co-treatments had similar sensitization effects, suggesting that MEF, THIO and FLU could similarly sensitize HAL-treated resistant cancer cells. Based on recent drug-screening results, AZA showed selective sensitization effects on cancer stem cells as MEF and THIO (13). Therefore, we tested whether AZA also increased the sensitivity of HAL-treated KBV20C cells. However, AZA did not increase the sensitivity of HAL-treated KBV20C cells (Figure 3C), whereas MEF and THIO sensitized HAL-treated KBV20C cells. These data suggest that P-gp-overexpressing resistant KBV20C cells respond differently to drugs compared to other cancer stem cells or the observed effects are specific to this cancer cell line. As shown in Figure 3C, verapamil presented high sensitization effects when combined with HAL, suggesting that P-gp inhibition is the most effective method to overcome HAL-resistance in cancer cells.
We did not observe differences in the sensitization effects among HAL-FLU, HAL-THIO and HAL-MEF using microscopic observations. We then used FACS analysis to observe which combination was the best to sensitize HAL-resistant KBV20C cells. As shown in Figure 4A and B, HAL-THIO presented a much better sensitization effect than HAL-MEF and HAL-FLU. HAL-MEF induced a similar G2 arrest and pre-G1 region when compared to HAL-FLU (Figure 4A and B). These results suggest that THIO has better sensitization effect on HAL-resistant cancer cells and that the best effect can be obtain by using lower concentration of THIO when combined with HAL. We also speculate that its toxicity can be lowered by using reduced THIO concentrations in combination with HAL. Collectively, highly resistant KBV20 cells can be sensitized by co-treatment with repositioned drugs, such as FLU, THIO and MEF.
Discussion
HAL has been recently developed and is especially promising for the treatment of patients for whom anti-cancer drugs previously failed (5-8, 24). Additional studies focusing on the mechanisms of HAL sensitization and toxicity would be beneficial for future studies examining the utility of HAL as a chemotherapeutic agent in various cancer types. Therefore, we aimed to increase our understanding of the mechanisms associated with sensitization of HAL-resistant KBV20C cancer cells that overexpress P-gp.
HAL-resistant KBV20C cells can be sensitized at HAL concentrations almost 500-fold higher than those required for parent sensitive KB cells, suggesting that HAL is effectively pumped out by P-gp. Considering that our previous studies showed less than 10-fold differences when using other antimitotic drugs (14, 17-19, 23, 25), these results also suggest that overexpressed P-gp can be an important mechanism of HAL resistance to avoid its toxicity. Since it is expected that HAL-treated cancer patients will ultimately develop a resistance to HAL, we assumed that our studies using highly HAL-resistant cancer cells could provide insight for the treatment of HAL-resistant cancer patients. In order to identify sensitization mechanisms, conditions or drugs, we tested repositioned drugs. Repositioned drugs, already used in the clinic, can be quickly applied without further toxic tests (26, 27). Based on a literature search and our previous studies (14, 17, 19, 23, 25), we tested whether HAL-treated KBV20C cells can be sensitized to HAL when co-treated with promising repositioned drugs, such as FLU, MEF, THIO and AZA.
We previously tested the sensitization effects of THIO and MEF (14, 19, 23). In this study, the effects of co-treatment using FLU and AZA were analyzed for the first time. Since both drugs showed similar sensitization effects in cancer screening and in the literature (13, 28, 29), we tested them and compared their effects to those of THIO and MEF. The cancer-sensitizing ability of FLU has been demonstrated in various cancer models (9-12, 28, 29), suggesting that it has potential as a chemotherapeutic agent. Single FLU treatment presented a similar IC50 between KB and KBV20C cells, indicating that FLU cannot be a target of P-gp. It also shows that single FLU treatment can be used in drug-resistant cancer cells without increasing FLU concentrations. Furthermore, we demonstrated that HAL-FLU co-treatment reduced cellular proliferation and increased G2 arrest in highly HAL-resistant KBV20C cells. Based on microscopic analysis, FACS analysis, annexin V analysis and western-blot analysis, we concluded that early stage apoptosis was increased by HAL-FLU co-treatment via increased G2 arrest and reduced proliferation. We hypothesize that FLU stimulates G2 arrest by increasing DNA damage, reducing cellular proliferation, thereby increasing apoptosis in HAL-treated resistant KBV20C cells. We also confirmed that FLU prevents the pumping-out ability of P-gp, acting as a weak P-gp inhibitor. Considering that single FLU treatment presented similar sensitization effects in P-gp-overexpressing resistant KBV20C and sensitive parent KB cells, we conclude that FLU sensitization mechanism involves both apoptosis and P-gp inhibition in HAL-treated resistant KBV20C cells.
THIO and MEF were previously shown to sensitize antimitotic drugs'-treated KBV20C cells (14, 19, 23). A repositioned drug, AZA, was recently shown to sensitize cancer stem cells or resistant cancer cells (13). Interestingly, the study showed that AZA, MEF and THIO selectively sensitized specific types of cancer stem cells (13). Therefore, we tested whether AZA, MEF and THIO could sensitize HAL-treated resistant KBV20C cells. FLU, MEF and THIO sensitized HAL-treated KBV20C cells, but AZA did not, suggesting that P-gp-overexpressing resistant KBV20C cells are different from the previously studied cancer stem cells. Furthermore, we assessed which drug presented the best sensitization effect in combination with HAL. Using FACS analysis, we observed that HAL-THIO combination treatment resulted in a better G2 arrest and pre-G1 region effects than HAL-MEF and HAL-FLU, indicating that THIO may be the best combination partner for HAL-resistant cancer treatment.
Altogether, our results show that highly HAL-resistant KBV20 cells can be killed by co-treatment with the repositioned drugs, FLU, THIO and MEF. Since these repositioned drugs are already used in clinical settings, the urgent need for pharmacological treatments of HAL-resistant cancers can be efficiently addressed and these drugs may be used to treat HAL-resistant patients at a relatively faster pace.
Acknowledgements
The Authors thank Ae-Ran Choi and Ju-Hwa Kim for help in technical supports and preparation of the manuscript. This research was supported by the National Research Foundation of Korea (NRF) funded by the Korean government (NRF-2016R1A4A1011189).
Footnotes
This article is freely accessible online.
Conflicts of Interest
The Authors declare no conflicts of interest.
- Received August 11, 2016.
- Revision received September 1, 2016.
- Accepted September 2, 2016.
- Copyright© 2016 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved