Effect of the Gc-derived macrophage-activating factor precursor (preGcMAF) on phagocytic activation of mouse peritoneal macrophages

Y Uto, S Yamamoto, R Takeuchi… - Anticancer …, 2011 - ar.iiarjournals.org
Background: The 1f1f subtype of the Gc protein (Gc1f1f protein) was converted into Gc-
derived macrophage-activating factor (GcMAF) by enzymatic processing in the presence of β …

β-Galactosidase treatment is a common first-stage modification of the three major subtypes of Gc protein to GcMAF

Y Uto, S Yamamoto, H Mukai, N Ishiyama… - Anticancer …, 2012 - ar.iiarjournals.org
Background: The 1f1f subtype of the group-specific component (Gc) protein is converted into
Gc protein-derived macrophage-activating factor (GcMAF) by enzymatic processing with β …

[PDF][PDF] Preparation of Gc protein-derived macrophage activating factor (GcMAF) and its structural characterization and biological activities

SB Mohamad, H Nagasawa, Y Uto, H Hori - Anticancer Research, 2002 - saisei-mirai.or.jp
Background: Gc protein has been reported to be a precursor of Gc protein-derived
macrophage activation factor (GcMAF) in the inflammation-primed macrophage activation …

Degalactosylated/desialylated human serum containing GcMAF induces macrophage phagocytic activity and in vivo antitumor activity

D Kuchiike, Y Uto, H Mukai, N Ishiyama… - Anticancer …, 2013 - ar.iiarjournals.org
Background: The group-specific component protein-derived macrophage-activating factor
(GcMAF) has various biological activities, such as macrophage activation and antitumor …

Characterization of human Gc protein-derived macrophage activation factor (GcMAF) and its functional role in macrophage tumoricidal activity

SB Mohamad, H Hori, H Nagasawa, K Usui… - Oxygen Transport To …, 2003 - Springer
Macrophages are essential for host defense and play an important role in orchestrating
immune response of the host against threat signals. Macrophages are also known to have a …

A novel assay system for macrophage-activating factor activity using a human U937 cell line

M Ishikawa, T Inoue, T Inui, D Kuchiike… - Anticancer …, 2014 - ar.iiarjournals.org
Background: Macrophages play important roles in antitumor immunity, and immunotherapy
with the group-specific component protein-derived macrophage-activating factor (GcMAF) …

[HTML][HTML] Simple method for large-scale production of macrophage activating factor GcMAF

Y Nabeshima, C Abe, T Kawauchi, T Hiroi, Y Uto… - Scientific Reports, 2020 - nature.com
Human group-specific component protein (Gc protein) is a multifunctional serum protein
which has three common allelic variants, Gc1F, Gc1S and Gc2 in humans. Gc1 contains an …

[PDF][PDF] Gc protein-derived macrophage activating factor (GcMAF): isoelectric focusing pattern and tumoricidal activity

SB Mohamad, H Nagasawa, H Sasaki, Y Uto… - Anticancer …, 2003 - saisei-mirai.or.jp
Background: Gc protein is the precursor for Gc proteinderived macrophage activating factor
(GcMAF), with three phenotypes: Gelf, Gels and Gc2, based on its elecrophoretic mobility …

Glycan structure of Gc protein-derived macrophage activating factor as revealed by mass spectrometry

CR Borges, DS Rehder - Archives of biochemistry and biophysics, 2016 - Elsevier
Disagreement exists regarding the O-glycan structure attached to human vitamin D binding
protein (DBP). Previously reported evidence indicated that the O-glycan of the Gc1S allele …

Gc protein-derived macrophage-activating factor (GcMAF) stimulates cAMP formation in human mononuclear cells and inhibits angiogenesis in chick embryo …

S Pacini, G Morucci, T Punzi, M Gulisano… - Cancer immunology …, 2011 - Springer
The effects of Gc protein-derived macrophage-activating factor (GcMAF) have been studied
in cancer and other conditions where angiogenesis is deregulated. In this study, we …