Significance of RNA reference in tumour-related gene expression analyses by cDNA array
N Laytragoon-Lewin, M Lagerlund… - Anticancer …, 2005 - ar.iiarjournals.org
The cDNA array technique is an efficient approach for studying the expression of a large
number of genes in a single experiment. The cDNA array analysis indicates the relative level …
number of genes in a single experiment. The cDNA array analysis indicates the relative level …
The impact of RNA standardization and heterogeneous gene expression on the results of cDNA array of human breast carcinoma
R Khoshnoud, Q He, M Sylván… - International …, 2010 - spandidos-publications.com
Abstract cDNA microarray is an established technique. However, difficulties such as
handling tissue samples under RNase-free conditions, the heterogeneous tumor …
handling tissue samples under RNase-free conditions, the heterogeneous tumor …
Tissue-specific selection of optimal reference genes for expression analysis of anti-cancer drug-related genes in tumor samples using quantitative real-time RT-PCR
H Wang, B Yang, T Geng, B Li, P Dai, C Chen - … and molecular pathology, 2015 - Elsevier
Gene transcription analysis in clinical tumor samples can help with diagnosis, prognosis,
and treatment of cancers. We aimed to identify the optimal reference genes for reliable …
and treatment of cancers. We aimed to identify the optimal reference genes for reliable …
Universal reference RNA is not a representative normal sample for oligonucleotide microarray studies
WD Mojica, L Stein, L Hawthorn - Pathology & Oncology Research, 2008 - Springer
Translational research has been defined as the scientific study using human material that
will ultimately generate patient specific data. A major caveat in human directed study is the …
will ultimately generate patient specific data. A major caveat in human directed study is the …
Core biopsies can be used to distinguish differences in expression profiling by cDNA microarrays
C Sotiriou, C Khanna, AA Jazaeri, D Petersen… - The Journal of molecular …, 2002 - Elsevier
The primary focus of this work was to determine the feasibility of obtaining representative
expression array profiles from clinical core biopsies. For this purpose we performed six 16 …
expression array profiles from clinical core biopsies. For this purpose we performed six 16 …
Comparative analysis of amplified and nonamplified RNA for hybridization in cDNA microarray
Limiting amounts of RNA is a major issue in cDNA microarray, especially when one is
dealing with fresh tissue samples. Here we describe a protocol based on template switch …
dealing with fresh tissue samples. Here we describe a protocol based on template switch …
[HTML][HTML] Optimization and evaluation of T7 based RNA linear amplification protocols for cDNA microarray analysis
Background T7 based linear amplification of RNA is used to obtain sufficient antisense RNA
for microarray expression profiling. We optimized and systematically evaluated the fidelity …
for microarray expression profiling. We optimized and systematically evaluated the fidelity …
[PDF][PDF] Total RNA-based target design for microarray analysis of defined tumor areas
R Dahse, A Berndt, H Kosmehl - BioTechniques, 2002 - Future Science
Efficient gene expression analysis at the cellular level using microarrays requires the
development and successful implementation of a variety of laboratory strategies. Major …
development and successful implementation of a variety of laboratory strategies. Major …
[HTML][HTML] Validation of cDNA microarray gene expression data obtained from linearly amplified RNA
SD Jenson, RS Robetorye, SD Bohling… - Molecular …, 2003 - ncbi.nlm.nih.gov
Background: DNA microarray technology has permitted the analysis of global gene
expression profiles for several diseases, including cancer. However, standard hybridisation …
expression profiles for several diseases, including cancer. However, standard hybridisation …
Construction of cDNA bank from biopsy specimens for multiple gene analysis of cancer
T Ishikawa, Y Ichikawa, Y Miura, M Momiyama… - Clinical …, 1997 - academic.oup.com
We constructed a “cDNA bank” of human colorectal cancer and surrounding normal tissues
with our unique mRNA assay system. Total nucleic acids extracted from patients' tissues …
with our unique mRNA assay system. Total nucleic acids extracted from patients' tissues …