Significance of RNA reference in tumour-related gene expression analyses by cDNA array

N Laytragoon-Lewin, M Lagerlund… - Anticancer …, 2005 - ar.iiarjournals.org
The cDNA array technique is an efficient approach for studying the expression of a large
number of genes in a single experiment. The cDNA array analysis indicates the relative level …

The impact of RNA standardization and heterogeneous gene expression on the results of cDNA array of human breast carcinoma

R Khoshnoud, Q He, M Sylván… - International …, 2010 - spandidos-publications.com
Abstract cDNA microarray is an established technique. However, difficulties such as
handling tissue samples under RNase-free conditions, the heterogeneous tumor …

Tissue-specific selection of optimal reference genes for expression analysis of anti-cancer drug-related genes in tumor samples using quantitative real-time RT-PCR

H Wang, B Yang, T Geng, B Li, P Dai, C Chen - … and molecular pathology, 2015 - Elsevier
Gene transcription analysis in clinical tumor samples can help with diagnosis, prognosis,
and treatment of cancers. We aimed to identify the optimal reference genes for reliable …

Universal reference RNA is not a representative normal sample for oligonucleotide microarray studies

WD Mojica, L Stein, L Hawthorn - Pathology & Oncology Research, 2008 - Springer
Translational research has been defined as the scientific study using human material that
will ultimately generate patient specific data. A major caveat in human directed study is the …

Core biopsies can be used to distinguish differences in expression profiling by cDNA microarrays

C Sotiriou, C Khanna, AA Jazaeri, D Petersen… - The Journal of molecular …, 2002 - Elsevier
The primary focus of this work was to determine the feasibility of obtaining representative
expression array profiles from clinical core biopsies. For this purpose we performed six 16 …

Comparative analysis of amplified and nonamplified RNA for hybridization in cDNA microarray

LI Gomes, RLA Silva, BS Stolf, EB Cristo… - Analytical …, 2003 - Elsevier
Limiting amounts of RNA is a major issue in cDNA microarray, especially when one is
dealing with fresh tissue samples. Here we describe a protocol based on template switch …

[HTML][HTML] Optimization and evaluation of T7 based RNA linear amplification protocols for cDNA microarray analysis

H Zhao, T Hastie, ML Whitfield, AL Børresen-Dale… - BMC genomics, 2002 - Springer
Background T7 based linear amplification of RNA is used to obtain sufficient antisense RNA
for microarray expression profiling. We optimized and systematically evaluated the fidelity …

[PDF][PDF] Total RNA-based target design for microarray analysis of defined tumor areas

R Dahse, A Berndt, H Kosmehl - BioTechniques, 2002 - Future Science
Efficient gene expression analysis at the cellular level using microarrays requires the
development and successful implementation of a variety of laboratory strategies. Major …

[HTML][HTML] Validation of cDNA microarray gene expression data obtained from linearly amplified RNA

SD Jenson, RS Robetorye, SD Bohling… - Molecular …, 2003 - ncbi.nlm.nih.gov
Background: DNA microarray technology has permitted the analysis of global gene
expression profiles for several diseases, including cancer. However, standard hybridisation …

Construction of cDNA bank from biopsy specimens for multiple gene analysis of cancer

T Ishikawa, Y Ichikawa, Y Miura, M Momiyama… - Clinical …, 1997 - academic.oup.com
We constructed a “cDNA bank” of human colorectal cancer and surrounding normal tissues
with our unique mRNA assay system. Total nucleic acids extracted from patients' tissues …