3D spheroid culture of hESC/hiPSC-derived hepatocyte-like cells for drug toxicity testing

…, Y Nagamoto, K Kishimoto, K Tashiro, F Sakurai… - Biomaterials, 2013 - Elsevier
Although it is expected that hepatocyte-like cells differentiated from human embryonic stem (ES)
cells or induced pluripotent stem (iPS) cells will be utilized in drug toxicity testing, the …

[HTML][HTML] Efficient generation of functional hepatocytes from human embryonic stem cells and induced pluripotent stem cells by HNF4α transduction

…, M Higuchi, K Tashiro, A Nonaka, F Sakurai… - Molecular Therapy, 2012 - cell.com
Hepatocyte-like cells from human embryonic stem cells (ESCs) and induced pluripotent
stem cells (iPSCs) are expected to be a useful source of cells drug discovery. Although we …

Prediction of interindividual differences in hepatic functions and drug sensitivity by using human iPS-derived hepatocytes

…, K Nishimura, K Imagawa, F Sakurai… - Proceedings of the …, 2014 - National Acad Sciences
Interindividual differences in hepatic metabolism, which are mainly due to genetic polymorphism
in its gene, have a large influence on individual drug efficacy and adverse reaction. …

Generation of metabolically functioning hepatocytes from human pluripotent stem cells by FOXA2 and HNF1α transduction

…, Y Nagamoto, H Watanabe, K Tashiro, F Sakurai… - Journal of …, 2012 - Elsevier
BACKGROUND & AIMS: Hepatocyte-like cells differentiated from human embryonic stem
cells (hESCs) and induced pluripotent stem cells (hiPSCs) can be utilized as a tool for …

Innate immune response induced by gene delivery vectors

H Sakurai, K Kawabata, F Sakurai, S Nakagawa… - International journal of …, 2008 - Elsevier
Gene therapy is a clinical strategy that has the potential to treat an array of genetic and
nongenetic diseases. Vectors for gene transfer are the essential tools of gene therapy. For gene …

Adenovirus vector-based vaccine for infectious diseases

F Sakurai, M Tachibana, H Mizuguchi - Drug Metabolism and …, 2022 - Elsevier
Replication-incompetent adenovirus (Ad) vectors have been widely used as gene delivery
vehicles in both gene therapy studies and basic studies for gene function analysis due to their …

Effect of DNA/liposome mixing ratio on the physicochemical characteristics, cellular uptake and intracellular trafficking of plasmid DNA/cationic liposome complexes …

F Sakurai, R Inoue, Y Nishino, A Okuda… - Journal of controlled …, 2000 - Elsevier
In order to identify the important factors involved in cationic liposome-mediated gene transfer,
in vitro transfection efficiencies by plasmid DNA complexed with DOTMA/DOPE liposomes …

[HTML][HTML] Transplantation of a human iPSC-derived hepatocyte sheet increases survival in mice with acute liver failure

…, K Takayama, K Ohashi, R Okamoto, F Sakurai… - Journal of …, 2016 - Elsevier
Background & Aims Hepatocyte transplantation is one of the most attractive approaches for
the treatment of patients with liver failure. Because human induced pluripotent stem cell-…

Efficient adipocyte and osteoblast differentiation from mouse induced pluripotent stem cells by adenoviral transduction

K Tashiro, M Inamura, K Kawabata, F Sakurai… - Stem …, 2009 - academic.oup.com
Induced pluripotent stem (iPS) cells, which are generated from somatic cells by transducing
four genes, are expected to have broad application to regenerative medicine. Although …

[HTML][HTML] Efficient generation of hepatoblasts from human ES cells and iPS cells by transient overexpression of homeobox gene HEX

…, K Kawabata, K Takayama, K Tashiro, F Sakurai… - Molecular Therapy, 2011 - cell.com
Human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) have the
potential to differentiate into all cell lineages, including hepatocytes, in vitro. Induced …