Cdc7 is an essential kinase that promotes DNA replication by activating origins of replication.
Here, we characterized the potent Cdc7 inhibitor PHA-767491 ( 1 ) in biochemical and cell-…

MPS1 kinase is a key regulator of the spindle assembly checkpoint (SAC), a mitotic mechanism
specifically required for proper chromosomal alignment and segregation. It has been …

Cdc7 is an evolutionarily conserved kinase that regulates S phase by promoting replication
origin activation. Down-regulation of Cdc7 by small interfering RNA in a variety of tumor cell …

Polo-like kinase 1 (PLK1) is a serine/threonine protein kinase considered to be the master
player of cell-cycle regulation during mitosis. It is indeed involved in centrosome maturation, …

Purpose: Aurora kinases play critical roles during mitosis in chromosome segregation and
cell division. The aim of this study was to determine the preclinical profile of a novel, highly …

The nuclear protein poly(ADP-ribose) polymerase-1 (PARP-1) has a well-established role
in the signaling and repair of DNA and is a prominent target in oncology, as testified by the …

Two small‐molecule–drug conjugates (SMDCs, 6 and 7) featuring lysosomally cleavable
linkers (namely the Val–Ala and Phe–Lys peptide sequences) were synthesized by …

Formation of pre-replicative complexes at origins is an early cell cycle event essential for
DNA duplication. A large body of evidence supports the notion that Cdc6 protein, through its …

High-throughput cell-based assays are becoming a powerful approach in the drug discovery
process. The ArrayScan™ high-content screening (HCS) reader is a cytometer based on a …

Polo-like kinase 1 (PLK1) is the master regulator of mitosis and a target for anticancer therapy.
To develop a marker of PLK1 activity in cells and tumour tissues, this study focused on …