Amifostine Increases FAS and Caspase-3 Expression in Colonic Tissue of Irradiated Mice

Anticancer Res. 2015 May;35(5):2817-22.

Abstract

Background/aim: The aim of this study was to evaluate the expression of FASL, FAS and FADD and caspase-3 in oesophagus, stomach and colonic tissues of mice irradiated in vivo by immunohistochemistry.

Materials and methods: A total of 48 adult male C57BL mice were distributed into four groups: Ami(-)/Rad(-): Mice received 0.5 ml of 0.9% physiological saline solution (PPS) intraperitioneally (i.p.); Ami(+)/Rad(-): mice received amifostine (400mg/kg i.p.) freshly dissolved in double-distilled water; Ami(-)/Rad(+): mice received 0.5 ml of PSS i.p. 30 min before a single whole-body radiation dose of 7 Gy; Ami(+)/Rad(+): mice received 0.5 ml of an aqueous solution of 400 mg/kg amifostine i.p.30 min prior to irradiation. All groups were assigned into subgroups sacrificed at 0.5 h, 1 h, 2 h and 4 h after irradiation.

Results: In oesophagus and stomach tissues, we did not observe any difference between Ami(-)/ad(-), Ami(+)/Rad(-), Ami(-)/Rad(+) and Ami(+)/Rad(+) groups in the expression of FASL, FAS and FADD. The colonic tissue was the only to exhibit any difference in the expression of FAS and caspase-3 protein in the Ami(-)/Rad(+)group at 1 and 2 h. Amifostine increased FAS and caspase-3 immunoexpression when compared to the control. Immunoexpression for FASL and FADD was not remarkably different in colonic tissue.

Conclusion: Taken together, our results demonstrate that amifostine increases FAS and caspase-3 expression in colonic tissue of irradiated mice.

Keywords: Amifostine; apoptosis; mouse; radiation.

MeSH terms

  • Amifostine / administration & dosage*
  • Animals
  • Caspase 3 / biosynthesis*
  • Colon / metabolism*
  • Colon / pathology
  • Colon / radiation effects
  • Gene Expression Regulation, Neoplastic / radiation effects
  • Humans
  • Mice
  • Radiation-Protective Agents / administration & dosage
  • Whole-Body Irradiation

Substances

  • Radiation-Protective Agents
  • Caspase 3
  • Amifostine