Recovery of nanogram quantities of DNA from plasma and quantitative measurement using labeling by nick translation

Anal Biochem. 1986 Nov 1;158(2):250-6. doi: 10.1016/0003-2697(86)90545-2.

Abstract

A method which allows for the quantitative measurement of DNA in plasma is described. After treatment of plasma with phenol, DNA is precipitated by ethanol using gelatin as a coprecipitating agent. DNA is then measured by nick translation labeling. This assay takes a few hours. It is suitable for the measurement of DNA within a range of 0.02 to 20 ng in 10 microliters of plasma. For example, it is applied to the measurement of DNA in plasma from mice injected with bacterial lipopolysaccharide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA / biosynthesis
  • DNA / blood*
  • Gelatin
  • Kinetics
  • Lipopolysaccharides / pharmacology
  • Mice
  • Microchemistry / methods
  • Polyethylene Glycols
  • Temperature

Substances

  • Lipopolysaccharides
  • Polyethylene Glycols
  • Gelatin
  • DNA