Abstract
MicroRNA-210 (miR-210), the master hypoxamir, plays pleiotropic roles in certain cancers; however, its role in the development of human colorectal cancer remains unclear. Herein, we report that miR-210 is frequently up-regulated in colorectal cancer tissues, with high miR-210 expression significantly correlating with large tumor size, lymph node metastasis, advanced clinical stage and poor prognosis. Functionally, miR-210 overexpression promotes the migration and invasion of colorectal cancer cells. Furthermore, miR-210 can be induced by hypoxia and mediates the hypoxia-induced metastasis of colorectal cancer cells. In addition, vacuole membrane protein 1 (VMP1) is identified as the direct and functional target of miR-210. Thus, miR-210 is a useful biomarker for hypoxic tumor cells and a prognostic factor that plays an essential role in colorectal cancer metastasis.
Publication types
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Research Support, Non-U.S. Gov't
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Retracted Publication
MeSH terms
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Blotting, Western
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Cell Line
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Cell Line, Tumor
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Colorectal Neoplasms / genetics*
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Colorectal Neoplasms / pathology*
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Gene Expression Regulation, Neoplastic / genetics
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Gene Expression Regulation, Neoplastic / physiology
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HT29 Cells
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Humans
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In Vitro Techniques
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Lymphatic Metastasis / genetics
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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MicroRNAs / genetics*
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Neoplasm Invasiveness / genetics
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Prognosis
Substances
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MIRN210 microRNA, human
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Membrane Proteins
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MicroRNAs
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VMP1 protein, human
Grants and funding
This study was supported by National Natural Science Foundation of China (No. 81271916; 81301506); Shandong Province Natural Science Foundation of China (No. ZR2010H004); Research Fund for the Doctoral Program of Higher Education of China (No. 20120131110055) and National Key Clinical Medical Specialties foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.