Abstract
The tumor microenvironment plays an important role in the progression of cancer. This study focused on carcinoma-associated fibroblasts (CAFs) and stromal-epithelial interaction between CAFs and epithelial ovarian carcinoma (EOC) cells. We isolated and established primary cultures of CAFs and co-cultured CAFs and EOC cells in vitro. The co-culture conditioned medium (CC-CM) was harvested and its influence on EOC cells was examined. Cytokine, chemokine, and growth factor levels were screened using a biotin label-based human antibody array system. We found that the stromal-epithelial crosstalk provided a suitable microenvironment for the progression of ovarian cancer cells in vitro.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis
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Cell Line, Tumor
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Cell Movement
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Cell Proliferation
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Chemokines / metabolism
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Coculture Techniques
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Culture Media, Conditioned / metabolism
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Cytokines / metabolism
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Disease Progression
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Epithelial Cells / metabolism*
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Epithelial Cells / pathology
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Female
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Fibroblasts / metabolism*
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Fibroblasts / pathology
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Humans
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Intercellular Signaling Peptides and Proteins / metabolism
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Neoplasm Invasiveness
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Ovarian Neoplasms / metabolism*
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Ovarian Neoplasms / pathology
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Paracrine Communication*
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Stromal Cells / metabolism*
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Stromal Cells / pathology
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Time Factors
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Tumor Microenvironment*
Substances
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Chemokines
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Culture Media, Conditioned
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Cytokines
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Intercellular Signaling Peptides and Proteins