Lipids in human prostate have been the subject of limited research and the most complete studies were published forty years ago. In this study we report on the total lipid, total cholesterol, free cholesterol and triglyceride content in human benign prostatic hypertrophy. By means of thin-layer chromatography, we separated the cholesterolesters in five fractions. The effect of treatment with anti-androgens before prostatectomy was examined.