Abstract
To investigate the role of zinc-finger protein 143 in cancer cells, we stably introduced ZNF143 expression knockdown by infecting colon cancer cells with short hairpin (sh) RNA-lentiviral particles against ZNF143 (HCT116 sh-ZNF143). Compared to sh-control cells, HCT116 sh-ZNF143 cells showed faster wound healing, increased migration through Transwell chambers, and increased invasion through Matrigel in Transwell chambers. ZNF143 knockdown increased transcriptional expression of ZEB1. Additionally, ZNF143 regulated E-cadherin transcriptional expression. Small interfering-RNA-mediated silencing of ZEB1 expression affected motility in HCT116 sh-ZNF143 cells. These data suggest that ZNF143 is involved in cellular motility through a ZEB1-E-cadherin-linked pathway in colon cancer cells.
Keywords:
E-cadherin; ZEB1; ZNF143; invasion; migration.
© 2013 Wiley Periodicals, Inc.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis
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Blotting, Western
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Cadherins / genetics
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Cadherins / metabolism*
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Cell Adhesion
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Cell Movement*
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Cell Proliferation
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Colonic Neoplasms / genetics
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Colonic Neoplasms / metabolism
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Colonic Neoplasms / pathology*
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Flow Cytometry
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Homeodomain Proteins / genetics
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Homeodomain Proteins / metabolism*
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Humans
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Immunoenzyme Techniques
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RNA, Messenger / genetics
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RNA, Small Interfering / genetics
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Real-Time Polymerase Chain Reaction
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Reverse Transcriptase Polymerase Chain Reaction
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Signal Transduction
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Trans-Activators / physiology*
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Tumor Cells, Cultured
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Wound Healing
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Zinc Finger E-box-Binding Homeobox 1
Substances
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Cadherins
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Homeodomain Proteins
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RNA, Messenger
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RNA, Small Interfering
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Trans-Activators
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Transcription Factors
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ZEB1 protein, human
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ZNF143 protein, human
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Zinc Finger E-box-Binding Homeobox 1