Abstract
We report a novel regulatory mechanism by which reactive oxygen species (ROS) regulate fumonisin B1 (FB1)-induced cell death. We found that FB1 induction of light-dependent ROS production promoted the degradation of GFP-labeled chloroplast proteins and increased phenylalanine ammonia lyase (PAL) activity, PAL1 gene expression and SA content, while pretreatment with ROS manipulators reversed these trends. Moreover, treatment with H2O2 or 3-amino-1,2,4-triazole increased PAL activity, PAL1 gene expression and SA content. PAL inhibitor significantly blocked FB1-induced lesion formation and SA increase. Our results demonstrate that light-dependent ROS accumulation stimulates the degradation of chloroplastic proteins and up-regulates PAL-mediated SA synthesis, thus promoting FB1-induced light-dependent cell death.
Copyright © 2013. Published by Elsevier B.V.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amitrole / pharmacology
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Arabidopsis / cytology
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Arabidopsis / drug effects
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Arabidopsis / metabolism*
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Arabidopsis / radiation effects
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Arabidopsis Proteins / antagonists & inhibitors
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism
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Cell Death / drug effects*
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Chloroplasts / drug effects
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Chloroplasts / metabolism*
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Chloroplasts / radiation effects
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Fumonisins / pharmacology*
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Gene Expression
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Hydrogen Peroxide / pharmacology
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Oxidants / pharmacology
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Phenylalanine Ammonia-Lyase / antagonists & inhibitors
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Phenylalanine Ammonia-Lyase / genetics
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Phenylalanine Ammonia-Lyase / metabolism
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Plant Leaves / cytology
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Plant Leaves / drug effects
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Plant Leaves / metabolism*
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Plant Leaves / radiation effects
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Proteolysis
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Reactive Oxygen Species / metabolism*
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Salicylic Acid / metabolism*
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Up-Regulation
Substances
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Arabidopsis Proteins
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Fumonisins
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Oxidants
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Reactive Oxygen Species
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fumonisin B1
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Hydrogen Peroxide
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Phenylalanine Ammonia-Lyase
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Salicylic Acid
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Amitrole