Abstract
Neuroendocrine tumors respond poorly to radiation and conventional chemotherapy, hence surgical removal of the neoplastic tissue is still the most effective way of treatment. In an attempt to find new therapeutic plant extracts of Christia vespertilionis (CV) their antitumor potential in human medullary thyroid carcinoma (MTC) and human small intestinal neuroendocrine tumor (SI-NET) cell lines were tested. Proliferation and viability were analyzed using cell counting and WST-1 assay. Apoptosis was determined by microscopy, luminescence assays for caspases 3/7, and expression studies of apoptosis-related genes. CV extracts showed antiproliferative and proapoptotic effects in all MTC and SI-NET cell lines, whereby high growth inhibition was observed by treatment with the ethylacetate-extracts (CV-45) in tumor cell lines but not in normal human fibroblasts. Furthermore CV-45 treatment resulted in alterations of gene expression of PDCD5, MTDH and TNFRSF10b in MTC as well as in SI-NET cells. The results indicate that Christia vespertilionis could serve as an anticancer therapeutic for treatment of neuroendocrine tumors.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents, Phytogenic / pharmacology*
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Apoptosis / drug effects
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Apoptosis Regulatory Proteins / genetics
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Apoptosis Regulatory Proteins / metabolism
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Caspase 3 / metabolism
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Caspase 7 / metabolism
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Cell Adhesion Molecules / genetics
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Cell Adhesion Molecules / metabolism
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Cell Line, Tumor / drug effects
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Cell Proliferation / drug effects*
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Cell Shape / drug effects
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Cell Survival / drug effects
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Drug Resistance, Neoplasm
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Fabaceae / chemistry*
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Gene Expression / drug effects
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Humans
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Membrane Proteins
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Neoplasm Proteins / genetics
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Neoplasm Proteins / metabolism
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Neuroendocrine Tumors / drug therapy*
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Plant Extracts / pharmacology*
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RNA-Binding Proteins
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Receptors, TNF-Related Apoptosis-Inducing Ligand / genetics
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Receptors, TNF-Related Apoptosis-Inducing Ligand / metabolism
Substances
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Antineoplastic Agents, Phytogenic
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Apoptosis Regulatory Proteins
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Cell Adhesion Molecules
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MTDH protein, human
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Membrane Proteins
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Neoplasm Proteins
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PDCD5 protein, human
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Plant Extracts
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RNA-Binding Proteins
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Receptors, TNF-Related Apoptosis-Inducing Ligand
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TNFRSF10B protein, human
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CASP3 protein, human
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CASP7 protein, human
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Caspase 3
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Caspase 7