Abstract
Hydrogen peroxide (H2O2) is upregulated in tumour microenvironments and may contribute to effects on metastatic cancer cells. This study demonstrates that treatment of lung carcinoma and melanoma cells with H2O2 for 14 days results in an induction of anoikis resistance and growth in an anchorage-independent condition. H2O2 exposure increased the Cav-1 (caveolin-1) level through an increase of Cav-1 mRNA with minimal effect on protein degradation. Upregulation of Cav-1 induced anoikis resistance and facilitated growth in a detached manner. The findings show a novel role of hydrogen peroxide in the regulation of metastatic potential of cancer cells.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Anoikis / drug effects*
-
Carcinoma, Non-Small-Cell Lung / metabolism
-
Carcinoma, Non-Small-Cell Lung / pathology*
-
Caveolin 1 / genetics
-
Caveolin 1 / metabolism
-
Cell Line, Tumor
-
Cell Survival
-
Gene Expression Regulation, Neoplastic*
-
Humans
-
Hydrogen Peroxide / pharmacology*
-
Lung / drug effects
-
Lung / metabolism
-
Lung / pathology
-
Melanoma / metabolism
-
Melanoma / pathology
-
Microscopy, Fluorescence
-
Proteolysis
-
RNA, Messenger / genetics
-
RNA, Messenger / metabolism
-
RNA, Small Interfering / genetics
-
RNA, Small Interfering / metabolism
-
Reactive Oxygen Species / metabolism
-
Time Factors
-
Transfection
-
Tumor Microenvironment
Substances
-
CAV1 protein, human
-
Caveolin 1
-
RNA, Messenger
-
RNA, Small Interfering
-
Reactive Oxygen Species
-
Hydrogen Peroxide