The chemoadjuvant potential of grape seed procyanidins on p53-related cell death in oral cancer cells

Yaoh-Shiang Lin; Su-Feng Chen; Chia-Lin Liu; Shin Nieh

doi:10.1111/j.1600-0714.2011.01103.x

The chemoadjuvant potential of grape seed procyanidins on p53-related cell death in oral cancer cells

J Oral Pathol Med. 2012 Apr;41(4):322-31. doi: 10.1111/j.1600-0714.2011.01103.x. Epub 2011 Nov 21.

Authors

Yaoh-Shiang Lin¹, Su-Feng Chen, Chia-Lin Liu, Shin Nieh

Affiliation

¹ Department of Otolaryngology-Head and Neck Surgery, National Defense Medical Centre & Tri-Service General Hospital, Taipei, Taiwan. yaohshiang@gmail.com

PMID: 22103929
DOI: 10.1111/j.1600-0714.2011.01103.x

Abstract

Background: To clarify the efficacy of grape seed procyanidin (GSP) on antiproliferative effects related to p53 functional status of oral squamous cell carcinoma (OSCC) for its chemoadjuvant potential.

Methods: We used GSP to investigate SCC-25 cells with wild-type p53 gene and OEC-M1 cells with mutant p53 gene for the assessment of antiproliferative effects including cell viability, cell cycle, apoptosis, migration and invasion potential, and alterations of associated oncoproteins involved in cellular and molecular events.

Results: The findings suggest that GSP on OEC-M1 cells leads to cell cycle arrest by increasing the expression of p21(Cip1) /p27(Kip1) protein without functioning mitochondria-mediated apoptosis, whereas GSP on SCC-25 cells inhibits cell proliferation via both G1-phase arrest and mitochondria-mediated apoptosis in a dose-dependent manner as a result of alterations of Bcl-2. GSP also inhibits the migration and invasion of both cells, which are associated with the suppression of matrix metalloproteinases (MMPs), MMP-2 and MMP-9.

Conclusion: Antiproliferative effectiveness of GSP is closely associated with the p53 status of OSCC cells. GSP displays chemoadjuvant potential via cell cycle blockage and apoptotic induction. Our findings clearly suggest that GSP may play a role as a novel chemopreventive or therapeutic agent for OSCC.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anticarcinogenic Agents / pharmacology
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects
Carcinoma, Squamous Cell / pathology*
Cell Cycle / drug effects
Cell Death / drug effects
Cell Line, Tumor
Cell Movement / drug effects
Cell Proliferation / drug effects
Cell Survival / drug effects
Cyclin-Dependent Kinase Inhibitor p21 / drug effects
Cyclin-Dependent Kinase Inhibitor p27 / drug effects
Dose-Response Relationship, Drug
G1 Phase / drug effects
Grape Seed Extract / pharmacology*
Humans
Matrix Metalloproteinase 2 / drug effects
Matrix Metalloproteinase 9 / drug effects
Mitochondria / drug effects
Mouth Neoplasms / pathology*
Neoplasm Invasiveness
Oncogene Proteins / drug effects
Plant Preparations / pharmacology*
Point Mutation / genetics
Proanthocyanidins / pharmacology*
Protein Kinase Inhibitors / analysis
Proto-Oncogene Proteins c-bcl-2 / drug effects
Tumor Suppressor Protein p53 / drug effects*
Tumor Suppressor Protein p53 / genetics
Vitis*

Substances

Anticarcinogenic Agents
Antineoplastic Agents
CDKN1A protein, human
Cyclin-Dependent Kinase Inhibitor p21
Grape Seed Extract
Grape Seed Proanthocyanidins
Oncogene Proteins
Plant Preparations
Proanthocyanidins
Protein Kinase Inhibitors
Proto-Oncogene Proteins c-bcl-2
TP53 protein, human
Tumor Suppressor Protein p53
Cyclin-Dependent Kinase Inhibitor p27
MMP2 protein, human
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9