Abstract
We present the first report of a T-helper cell line, HUT 102, constitutively producing both granulocyte-macrophage colony-stimulating factor (GM-CSF) and monocytic colony-stimulating factor (M-CSF), as detected by sensitive enzyme-linked immunosorbent assays and Northern blot analysis. However, neither amplification nor structural change of the GM-CSF and M-CSF genes was detected by Southern blot analysis. In the case of HUT 102, in which human T-lymphotropic leukemia virus type I (HTLV-I) is integrated, the viral protein, which acts as a trans-acting transcriptional activator, may induce the production of both GM-CSF and M-CSF.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line
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DNA Probes
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Deoxyribonuclease BamHI
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Deoxyribonuclease HindIII
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Enzyme-Linked Immunosorbent Assay
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Granulocyte-Macrophage Colony-Stimulating Factor / biosynthesis*
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Granulocyte-Macrophage Colony-Stimulating Factor / genetics
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Humans
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Macrophage Colony-Stimulating Factor / biosynthesis*
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Macrophage Colony-Stimulating Factor / genetics
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Nucleic Acid Hybridization
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T-Lymphocytes, Helper-Inducer / metabolism*
Substances
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DNA Probes
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Macrophage Colony-Stimulating Factor
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Granulocyte-Macrophage Colony-Stimulating Factor
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Deoxyribonuclease BamHI
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Deoxyribonuclease HindIII