Boron neutron capture therapy induces cell cycle arrest and DNA fragmentation in murine melanoma cells

F Faião-Flores; P R P Coelho; J Arruda-Neto; Durvanei A Maria

doi:10.1016/j.apradiso.2011.03.005

Boron neutron capture therapy induces cell cycle arrest and DNA fragmentation in murine melanoma cells

Appl Radiat Isot. 2011 Dec;69(12):1741-4. doi: 10.1016/j.apradiso.2011.03.005. Epub 2011 Mar 21.

Authors

F Faião-Flores¹, P R P Coelho, J Arruda-Neto, Durvanei A Maria

Affiliation

¹ Biochemical and Biophysical Laboratory, Butantan Institute, São Paulo, Brazil.

PMID: 21441034
DOI: 10.1016/j.apradiso.2011.03.005

Abstract

The melanoma is a highly lethal skin tumor, with a high incidence. Boron Neutron Capture Therapy (BNCT) is a radiotherapy which combines Boron with thermal neutrons, constituting a binary system. B16F10 melanoma and L929 fibroblasts were treated with Boronophenylalanine and irradiated with thermal neutron flux. The electric potential of mitochondrial membrane, cyclin D1 and caspase-3 markers were analyzed. BNCT induced a cell death increase and cyclin D1 amount decreased only in B16F10 melanoma. Besides, there was not caspase-3 phosphorylation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Boron Neutron Capture Therapy*
Caspase 3 / metabolism
Cell Cycle Checkpoints*
Cell Line, Tumor
DNA Fragmentation*
Melanoma, Experimental / pathology
Melanoma, Experimental / radiotherapy*
Mice

Substances

Caspase 3