Chemotherapy with hybrid liposomes for acute lymphatic leukemia leading to apoptosis in vivo

Int J Pharm. 2011 Mar 15;406(1-2):173-8. doi: 10.1016/j.ijpharm.2010.12.041. Epub 2011 Jan 8.

Abstract

Hybrid liposomes (HL) composed of 95 mol% l-α-dimyristoylphosphatidylcholine (DMPC) and 5 mol% polyoxyethylene (25) dodecyl ether (C(12)(EO)(25)) were prepared by sonication. A clear solutions of HL-25 having hydrodynamic diameter of about 50 nm could be maintained over 3 weeks. Remarkable reduction of tumor volume in model mice of acute lymphatic leukemia (ALL) intravenously treated with HL-25 without drugs after the subcutaneously inoculation of human ALL (MOLT-4) cells was verified in vivo. Induction of apoptosis in tumor of model mice of ALL treated with HL-25 was observed in micrographs on the basis of TUNEL method. Remarkable decrease of the ascites in ALL model mice treated with HL-25 was observed. It is noteworthy that prolonged survival (>400%) was obtained in model mice of ALL after the treatment with HL-25 without drugs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects*
  • Cell Culture Techniques
  • Cell Line, Tumor
  • Dimyristoylphosphatidylcholine / administration & dosage
  • Dimyristoylphosphatidylcholine / chemistry
  • Dimyristoylphosphatidylcholine / therapeutic use*
  • Female
  • Humans
  • In Situ Nick-End Labeling
  • Liposomes
  • Membrane Potential, Mitochondrial / drug effects
  • Mice
  • Mice, SCID
  • Polyethylene Glycols / administration & dosage
  • Polyethylene Glycols / chemistry
  • Polyethylene Glycols / therapeutic use*
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / drug therapy*
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / pathology
  • Survival Analysis
  • Xenograft Model Antitumor Assays

Substances

  • Liposomes
  • polyoxyethylene(25)dodecyl ether
  • Polyethylene Glycols
  • Dimyristoylphosphatidylcholine