Abstract
Extensive data indicate that miR-21 plays a critical role in gliomagenesis, however, knowledge is limited on the mechanism of action of miR-21, including cell proliferation, apoptosis, and migration. In this study, we showed that down-regulation of miR-21 expression by antisense oligonucleotides inhibited glioma cell proliferation and induced cell apoptosis. Moreover, reduction of miR-21 activated caspase 9 and 3, which may be mediated by modulating multiple potential target genes, such as TIMP3. Together, these findings indicate that miR-21 plays a key role in regulating cell apoptosis in gliomas and may serve as a target for effective therapies.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apoptosis / drug effects*
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Apoptosis / genetics
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Brain Neoplasms / drug therapy
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Brain Neoplasms / genetics
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Brain Neoplasms / metabolism
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Brain Neoplasms / pathology*
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Caspase 3 / metabolism*
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Caspase 9 / metabolism*
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Cell Line, Tumor
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Down-Regulation / drug effects
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Down-Regulation / physiology
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Enzyme Activation / drug effects
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Gene Expression Regulation, Neoplastic / drug effects
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Glioma / drug therapy
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Glioma / genetics
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Glioma / metabolism
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Glioma / pathology*
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Humans
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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MicroRNAs / antagonists & inhibitors*
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MicroRNAs / genetics
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Oligodeoxyribonucleotides, Antisense / pharmacology*
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Oligodeoxyribonucleotides, Antisense / therapeutic use
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Xenograft Model Antitumor Assays
Substances
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MIRN21 microRNA, human
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MicroRNAs
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Oligodeoxyribonucleotides, Antisense
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Caspase 3
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Caspase 9