In vivo investigation of CD133 as a putative marker of cancer stem cells in Hep-2 cell line

Head Neck. 2009 Jan;31(1):94-101. doi: 10.1002/hed.20935.

Abstract

Background: Mounting evidence suggests that most tumors consist of a heterogeneous population of cells with a subset population that has the exclusive tumorigenic ability. They are called cancer stem cells (CSCs). CSCs can self-renew to generate additional CSCs and also differentiate to generate phenotypically diverse cancer cells with limited proliferative potential. They have been identified in a variety of tumors. In this study, we identify the marker of CSCs in the established human laryngeal tumor Hep-2 cell line in vivo. Our in vitro experiment shown as CD133, a 5-transmembrane glycoprotein expressed in Hep-2 cell line. CD133 was supposed as a candidate of CSC in laryngeal carcinoma. In this study, the expression of CD133 was detected in a Hep-2 cell line. Applying the magnetic cell sorting (MACS) technology, we reported the results of purifying CD133 positive cells from a Hep-2 cell line. Three-type cells' tumor-forming ability was examined in vivo to identify the marker of CSCs in Hep-2 cell line.

Methods: CD133 was selected as a putative marker of CSC in laryngeal carcinoma, Hep-2 cell lines. Flow cytometry was used to detect the expression of CD133 in the Hep-2 cell line. Immunomagnetic beads were applied to purify CD133-positive cells. CD133(+), CD133(-) tumor cells, and unsorted Hep-2 cells were injected into severe combined immune deficiency (SCID) mice individually to observe tumor-forming ability.

Results: Only a small proportion (3.15% +/- 0.83%) of cells in the Hep-2 cell line express the CD133 marker. In comparison with CD133(-) tumor cells and unsorted cells, CD133(+) cells possess a marked capacity for tumor formation in vivo (p <.05).

Conclusion: CD133 is 1 of the markers for CSCs in human laryngeal tumors of the Hep-2 cell line. Work on the characterization of these cells provides a powerful tool to investigate the tumorigenic process in the larynx and to develop therapies targeting the CSC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AC133 Antigen
  • Antigens, CD / isolation & purification
  • Antigens, CD / metabolism*
  • Cell Cycle
  • Cell Line, Tumor
  • Flow Cytometry
  • Glycoproteins / isolation & purification
  • Glycoproteins / metabolism*
  • Humans
  • Immunohistochemistry
  • Immunomagnetic Separation
  • Laryngeal Neoplasms / metabolism*
  • Neoplastic Stem Cells / metabolism*
  • Peptides / isolation & purification
  • Peptides / metabolism*

Substances

  • AC133 Antigen
  • Antigens, CD
  • Glycoproteins
  • PROM1 protein, human
  • Peptides
  • Prom1 protein, mouse