Abstract
A novel lymphoma cell line, designated TMD8 was established from cells of a patient with diffuse large B-cell lymphoma. TMD8 cells expressed HES1 mRNA, suggesting constitutive activation of Notch signaling. TMD8 cells expressed normal-sized Notch1 protein, and showed no mutations in the NOTCH1 gene. Cell growth was suppressed by gamma-secretase inhibitors (GSI). It was reported that GSI suppressed growth of T-cell acute lymphoblastic leukemia (T-ALL) cell lines, which frequently had NOTCH1 mutations. In addition to T-ALL, TMD8 is another unique cell line sensitive to GSI, and is useful to study effects of GSI in molecular targeting therapy.
Publication types
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Case Reports
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing
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Amyloid Precursor Protein Secretases / antagonists & inhibitors*
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Basic Helix-Loop-Helix Transcription Factors / drug effects
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Basic Helix-Loop-Helix Transcription Factors / genetics
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Calcium-Binding Proteins
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Cell Line, Tumor*
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Cell Proliferation / drug effects*
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Cell Survival / drug effects
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Dipeptides / pharmacology*
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Enzyme Inhibitors / pharmacology*
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Fatal Outcome
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Homeodomain Proteins / drug effects
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Homeodomain Proteins / genetics
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Humans
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Intercellular Signaling Peptides and Proteins / genetics
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Jagged-2 Protein
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Karyotyping
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Ligands
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Lymphoma, B-Cell / drug therapy*
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Lymphoma, B-Cell / genetics
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Lymphoma, B-Cell / pathology*
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Male
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Membrane Proteins / genetics
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Middle Aged
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Oligopeptides / pharmacology*
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RNA, Messenger / drug effects
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RNA, Messenger / genetics
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Receptor, Notch1 / genetics
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Receptor, Notch2 / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Structure-Activity Relationship
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Transcription Factor HES-1
Substances
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Adaptor Proteins, Signal Transducing
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Basic Helix-Loop-Helix Transcription Factors
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Calcium-Binding Proteins
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DLL4 protein, human
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Dipeptides
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Enzyme Inhibitors
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Homeodomain Proteins
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Intercellular Signaling Peptides and Proteins
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JAG2 protein, human
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Jagged-2 Protein
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Ligands
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Membrane Proteins
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N-(N-(3,5-difluorophenacetyl)alanyl)phenylglycine tert-butyl ester
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NOTCH1 protein, human
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NOTCH2 protein, human
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Oligopeptides
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RNA, Messenger
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Receptor, Notch1
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Receptor, Notch2
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Transcription Factor HES-1
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benzyloxycarbonyl-isoleucyl-leucinal
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benzyloxycarbonyl-leucyl-leucyl-norleucinal
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HES1 protein, human
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Amyloid Precursor Protein Secretases