The effect of quercetin on pro-apoptotic activity of cisplatin in HeLa cells

J Jakubowicz-Gil; R Paduch; T Piersiak; K Głowniak; A Gawron; M Kandefer-Szerszeń

doi:10.1016/j.bcp.2005.01.022

The effect of quercetin on pro-apoptotic activity of cisplatin in HeLa cells

Biochem Pharmacol. 2005 May 1;69(9):1343-50. doi: 10.1016/j.bcp.2005.01.022.

Authors

J Jakubowicz-Gil¹, R Paduch, T Piersiak, K Głowniak, A Gawron, M Kandefer-Szerszeń

Affiliation

¹ Department of Comparative Anatomy and Anthropology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland. jjgil@biotop.umcs.lublin.pl

PMID: 15826605
DOI: 10.1016/j.bcp.2005.01.022

Abstract

It is well known that some tumour cells are very resistant to chemotherapy-induced cell death which indicate poor prognosis for patients. Thus the aim of the present study was to investigate the effect of quercetin on pro-apoptotic activity of cisplatin in human cervix carcinoma cells (HeLa). Three variants of experiments were performed. In the first one cells were incubated with studied drugs separately for 8 and 24h. In the second, drugs were added to the culture medium simultaneously. In third cisplatin or quercetin addition was followed by subsequent quercetin or cisplatin treatment, respectively. We observed different apoptotic effects, dependent on the drug succession. Preincubation of cells with quercetin followed by cisplatin treatment appeared to be the most effective and was correlated with strong activation of caspase-3 and inhibition of both heat shock proteins (Hsp72) and multi-drug resistance proteins (MRP) levels. Our results indicate that quercetin pretreatment sensitizes HeLa cells to cisplatin-induced apoptosis in HeLa cells.

MeSH terms

Antineoplastic Agents / antagonists & inhibitors
Antineoplastic Agents / pharmacology*
Antineoplastic Agents, Phytogenic / antagonists & inhibitors
Antineoplastic Agents, Phytogenic / pharmacology
Benzimidazoles
Blotting, Western
Caspase 3
Caspases / drug effects
Caspases / metabolism
Cisplatin / antagonists & inhibitors
Cisplatin / pharmacology*
Drug Interactions
Drug Resistance, Neoplasm
Drug Therapy, Combination
Electrophoresis, Polyacrylamide Gel
Enzyme Activation / drug effects
Fluorescent Antibody Technique, Indirect
Fluorescent Dyes
HeLa Cells
Heat-Shock Proteins / antagonists & inhibitors
Heat-Shock Proteins / drug effects
Humans
Neoplasm Proteins / drug effects*
Neoplasm Proteins / metabolism
Neoplasms / drug therapy*
Neoplasms / metabolism
Quercetin / antagonists & inhibitors
Quercetin / pharmacology*
Time Factors

Substances

Antineoplastic Agents
Antineoplastic Agents, Phytogenic
Benzimidazoles
Fluorescent Dyes
Heat-Shock Proteins
Neoplasm Proteins
Quercetin
CASP3 protein, human
Caspase 3
Caspases
bisbenzimide ethoxide trihydrochloride
Cisplatin