Abstract
Objective:
We established a new monoclonal antibody (2C9) that reacted with prostate tissue. The immunohistochemical reactivity of this antibody is similar to anti-prostate-specific membrane antigen (PSMA). Herein, we report the antigenic determinant of 2C9 antibody.
Methods:
The reactivity of the antibody was characterized by immunohistochemical staining and the antigen target was characterized by amino acid sequencing after immuno-affinity purification from an LNCaP cell lysate and cloning of a cDNA using a mammalian expression cDNA cloning system.
Results:
The amino acid and nucleotide sequences for the antigen molecule recognized with 2C9 monoclonal antibody demonstrated identity with PSMA.
Conclusion:
The target molecule of the 2C9 monoclonal antibody is PSMA, pointing to future diagnostic and therapeutic applications.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Antibodies, Monoclonal / chemistry
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Antibodies, Monoclonal / immunology*
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Antibodies, Monoclonal / isolation & purification
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Base Sequence
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Biomarkers, Tumor / genetics
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Biomarkers, Tumor / immunology*
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Blotting, Northern
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Cell Line, Tumor / immunology
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Cell Line, Tumor / metabolism
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Clone Cells / immunology
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DNA, Complementary / genetics
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DNA, Complementary / isolation & purification
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Epitope Mapping / methods
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Epitopes / analysis*
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Humans
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Male
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Molecular Sequence Data
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Molecular Weight
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Prostate / chemistry
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Prostate / immunology
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Prostate-Specific Antigen / analysis*
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Prostate-Specific Antigen / chemistry
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Prostatic Neoplasms / chemistry
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Prostatic Neoplasms / genetics
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Prostatic Neoplasms / immunology*
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Sequence Analysis, Protein
Substances
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Antibodies, Monoclonal
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Biomarkers, Tumor
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DNA, Complementary
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Epitopes
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Prostate-Specific Antigen