Abstract
We analyzed all 21 exons of the RET proto-oncogene of paired genomic DNA from tumors and normal tissues in 12 small cell lung cancer (SCLC) patients for the presence of genetic alteration. Polymerase chain reaction single-strand conformation polymorphism analysis and direct sequencing revealed that heterozygosity of the RET proto-oncogene was lost in the tumor tissues of six patients out of eight informative SCLC patients, although point mutation was not evident in any tumors. These results suggest that a deletion of the chromosomal region including the RET proto-oncogene is involved in the pathogenesis of SCLC.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carcinoma, Small Cell / genetics*
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Chromosomes, Human, Pair 10 / genetics*
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DNA Mutational Analysis
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DNA, Neoplasm / genetics*
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Exons / genetics
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Gene Deletion*
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Humans
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Introns / genetics
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Lung Neoplasms / genetics*
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Polymerase Chain Reaction
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Polymorphism, Single-Stranded Conformational
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Proto-Oncogene Mas
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Proto-Oncogene Proteins / deficiency
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogene Proteins c-ret
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Receptor Protein-Tyrosine Kinases / deficiency
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Receptor Protein-Tyrosine Kinases / genetics*
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Sequence Analysis, DNA
Substances
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DNA, Neoplasm
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MAS1 protein, human
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Proto-Oncogene Mas
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-ret
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Receptor Protein-Tyrosine Kinases