Abstract
Thirty-seven naturally occurring withanolides (1-37), previously isolated in our laboratories, were evaluated for their potential to induce quinone reductase with cultured murine hepatoma cells (Hepa 1c1c7). Spiranoid (29, 32) and 18-functionalized withanolides (2-5, 7-9, 24) were found to be potent inducers of the enzyme, while 5alpha-substituted derivatives exhibited weak activity. Preliminary studies were performed with compound 29 to evaluate enzyme-inducing capacity in multiple organ sites of BALB/c mice. Significant induction was observed in liver and colon, but not in lung, stomach, or mammary gland.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Brassicaceae / chemistry*
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Breast / enzymology
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Carcinoma, Hepatocellular / enzymology*
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Colon / enzymology
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Enzyme Induction / drug effects
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Inhibitory Concentration 50
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Liver / enzymology
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Lung / enzymology
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Mice
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Mice, Inbred BALB C
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Molecular Structure
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Nuclear Magnetic Resonance, Biomolecular
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Plants, Medicinal / chemistry*
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Quinone Reductases / biosynthesis*
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Solanaceae / chemistry*
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Stereoisomerism
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Steroids / chemistry*
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Steroids / isolation & purification
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Steroids / pharmacology
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Stilbenes / pharmacology*
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Stomach / enzymology
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Tumor Cells, Cultured / drug effects
Substances
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Steroids
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Stilbenes
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Quinone Reductases