Abstract
Although apoptosis plays an essential role in the embryogenesis and homeostasis of multicellular organisms, this mechanism has not yet been fully clarified. We isolated a novel human apoptosis-inducing gene, ASY, which encodes an endoplasmic reticulum-targeting protein without any known apoptosis-related motifs. This gene is identical to the Nogo-B, a splice variant of the Nogo-A which has recently been shown to be an inhibitor of neuronal regeneration in the central nervous system. Ectopic expression of the ASY gene led to extensive apoptosis, particularly in cancer cells. Furthermore, transcription of the ASY gene was suppressed in small cell lung cancer. These results suggest that a new type of apoptosis-inducing gene, namely, ASY, may be involved in the development of certain types of cancer.
MeSH terms
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Amino Acid Motifs
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Amino Acid Sequence
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Apoptosis / genetics*
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Bone Neoplasms / metabolism
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Bone Neoplasms / pathology
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Carcinoma, Small Cell / genetics
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Carcinoma, Small Cell / metabolism
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DNA, Complementary / genetics
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Endoplasmic Reticulum / metabolism
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Fibroblasts / metabolism
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Fibroblasts / pathology
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Fibrosarcoma / metabolism
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Fibrosarcoma / pathology
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Genes, Regulator*
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HeLa Cells / metabolism
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HeLa Cells / pathology
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Humans
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Hybrid Cells / metabolism
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Hybrid Cells / pathology
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Lung Neoplasms / genetics
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Lung Neoplasms / metabolism
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Melanoma / metabolism
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Melanoma / pathology
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Molecular Sequence Data
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Myelin Proteins / biosynthesis
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Myelin Proteins / genetics*
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Neoplasm Proteins / biosynthesis
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Neoplasm Proteins / genetics*
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Neoplasms / genetics*
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Nogo Proteins
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Osteosarcoma / metabolism
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Osteosarcoma / pathology
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RNA Splicing
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RNA, Messenger / genetics
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RNA, Neoplasm / genetics
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Transfection
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Tumor Cells, Cultured / metabolism
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Tumor Cells, Cultured / pathology
Substances
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DNA, Complementary
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Myelin Proteins
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Neoplasm Proteins
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Nogo Proteins
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RNA, Messenger
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RNA, Neoplasm
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RTN4 protein, human