Abstract
VAV3 is a new member of the VAV oncogene family with a strong homology to VAV and VAV2. A conceptual translation of the cDNA indicates that VAV3 is between 40 and 77% identical to VAV and VAV2 at the amino acid level in all identified functional motifs. This homology suggests that VAV3 occupies a similar position in signal transduction as the other family members. A major variant transcript, VAV3.1, found in both humans and mice, appears to encode only the 3' SH3-SH2-SH3 region, which suggests that it may substitute for the full-length isoform in functions mediated by this domain, or compete with the full-length isoform in functions mediated by more N-terminal motifs. VAV3.1 either is a partly unspliced mRNA or originates from a different promoter. VAV3 transcripts are found in cells of hematopoietic origin, where VAV is primarily expressed. However, unlike, VAV, the VAV3 and VAV3.1 transcripts are also found at varying levels in a wide variety of other tissues and cell lines. TGF-beta and EGF reversibly down-regulate the abundance of the VAV3. 1 transcript in HaCaT keratinocytes, representing the first observation of transcript regulation of a member of the VAV family by a growth factor.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Cycle Proteins*
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Cell Line
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Cloning, Molecular
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Culture Media / pharmacology
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Culture Media, Serum-Free / pharmacology
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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Epidermal Growth Factor / pharmacology
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Female
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Gene Expression Regulation / drug effects
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Genetic Variation
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Guanine Nucleotide Exchange Factors
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HT29 Cells
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HeLa Cells
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Humans
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Male
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Mice
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Molecular Sequence Data
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Oncogene Proteins / genetics*
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Protein Isoforms / genetics
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Protein Isoforms / physiology
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogene Proteins / physiology
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Proto-Oncogene Proteins c-vav
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RNA / drug effects
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RNA / genetics
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RNA / metabolism
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
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Tetradecanoylphorbol Acetate / pharmacology
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Tissue Distribution
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Transcription, Genetic
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Transforming Growth Factor beta / pharmacology
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Tumor Cells, Cultured
Substances
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Cell Cycle Proteins
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Culture Media
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Culture Media, Serum-Free
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DNA, Complementary
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Guanine Nucleotide Exchange Factors
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Oncogene Proteins
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Protein Isoforms
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-vav
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Transforming Growth Factor beta
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VAV1 protein, human
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VAV3 protein, human
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Vav1 protein, mouse
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Vav3 protein, mouse
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Epidermal Growth Factor
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RNA
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Tetradecanoylphorbol Acetate
Associated data
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GENBANK/AF035442
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GENBANK/AF067816
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GENBANK/AF067817
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GENBANK/AF140280