Biodistribution of Radiolabeled Adenosylcobalamin in Patients Diagnosed With Various Malignancies
Section snippets
Patients
After receiving approval from the Mayo Clinic Institutional Review Board and the Radioactive Drug Research Committee, we enrolled 30 patients with radiographically or clinically diagnosed breast, lung, colon, sarcomatous,
Synthesis and Analysis of DAC
The synthesis of DAC has been described previously.2 Mass spectrometry (MS) data were obtained on a Sciex Atmospheric Pressure Ionisation 365 mass spectrometer (Toronto, Ontario). Separations were carried out using online high-pressure liquid chromatography (HPLC) consisting of
Patients
Patients were enrolled over a 9-month interval (January-September 1999). Of the 30 patients studied, 14 were women and 16 were men. The median age (range) was 59.9 years (36–80 years) for the women and 56.4 years (19–81 years) for the men. Of the women studied, 9 were evaluated for breast cancer, 2 for sarcomatous lesions (leiomyosarcoma, osteogenic sarcoma), 2 for thyroid malignancies (anaplastic, medullary), and 1 for presumed lung cancer. Of the 16 men enrolled, 4 had presumed lung cancers,
Overview of Vitamin B12 Chemistry
The colored organometallic tetrapyrole compounds are essential for life.3 Pernicious anemia or lack of vitamin B12 in humans is a case in point. Adenosylcobalamin is the mitochondrial coenzyme for methylmalonyl-coenzyme A (CoA) mutase. This coenzyme is ultimately responsible for the conversion of the amino acids methionine, threonine, isoleucine, and valine, any odd chain fatty acids, and proprionyl CoA to succinyl CoA. Subsequently succinyl CoA enters into the tricarboxylic acid (Krebs) cycle
Conclusions
The findings in the first 30 patients studied with In 111 DAC demonstrated that the biodistribution of the vitamin B12 analog was similar between the sexes. There is rapid serum clearance and marked urinary excretion of the analog. The optimal imaging time for tumors appears to be 3 to 5 hours after injection of the tracer, with the exception of 24 hours for primary CNS tumors, advanced metastatic prostate disease, and Htirthle cell thyroid cancer. In the various types of malignancies imaged,
Acknowledgment
The authors thank Rose M. Busta for preparation of the manuscript.
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This study was supported in part by the Mayo Foundation and the University of Minnesota.