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Aquaporin 1 Is Overexpressed in Lung Cancer and Stimulates NIH-3T3 Cell Proliferation and Anchorage-Independent Growth

https://doi.org/10.2353/ajpath.2006.050596Get rights and content

The aquaporins represent a family of transmembrane water channel proteins that play a major role in trans-cellular and transepithelial water movement. Most tumors have been shown to exhibit high vascular permeability and interstitial fluid pressure, but the transport pathways for water within tumors remain unknown. Here, we tested 10 non-small cell lung cancer cell lines of various origins by reverse transcriptase-polymerase chain reaction and Western blot analysis and identified clear expression of aquaporin 1 (AQP1) in seven cell lines. We next examined the distribution of the AQP1 protein in several types of primary lung tumors (16 squamous cell carcinomas, 21 adenocarcinomas, and 7 bronchoalveolar carcinomas) by immunohistochemical staining. AQP1 was overexpressed in 62% (13 of 21) and 75% (6 of 8) of adenocarcinoma and bronchoalveolar carcinoma, respectively, whereas all cases of squamous cell carcinoma and normal lung tissue were negative. Forced expression of full-length AQP1 cDNA in NIH-3T3 cells induced many phenotypic changes characteristic of transformation, including cell proliferation-enhancing activity by the MTT assay and anchorage-independent growth in soft agar. Although further details on the molecular function of AQP1 related to tumorigenesis remain to be elucidated, our results suggest a potential role of AQP1 as a novel therapeutic target for the management of lung cancer.

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Supported in part by the National Cancer Institute Specialized Programs of Research Excellence (grant P50 CA96784-01 to C.M.), the American Cancer Society (grant RPG-98-054 to L.M.), the Fondation de France, AP-HP (to J-C.S.), the Lilly Fondation (to J-C.S.), Cancer Center (grant P30 CA16620 to M. D. Anderson Cancer Center), the Tobacco Research Fund from the State of Texas (to M. D. Anderson Cancer Center), Cancer research grant from Pyung-Ya Foundation (to C.M.), and a research grant from the Korea Science and Engineering Foundation (to S.J.J.).

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