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Histological, immunohistochemical, ultrastructural and biochemical study of human gastric composite tumor: Expression of the serotonin-2B receptor by the neuroendocrine component

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Abstract

We report a case of a human gastric composite tumor occurring seven years after a partial gastrectomy for a low grade B cell MALT lymphoma. Histological examination of the tumor revealed two intimately intermingled components:

  1. 1.

    A moderately to poorly differentiated tubulo-acinar adenocarcinoma with signetring cells; and

  2. 2.

    Isolated or clustered small neuroendocrine cells without atypia expressing chromogranin A, somatostatin and/or glucagon, serotonin (5-HT) and, the 5-HT2B receptors. In addition to immunohistochemical detection, the presence of 5-HT2B receptors was shown pharmacologically through [125l]-DOI binding.

Since 5-HT2B receptors have been demonstrated to have autocrine functions and, mitogenic and transforming properties, these results suggest a role of 5-HT in neuroendocrine malignant transformation. On the other hand, the expression of somatostatin and the detection by reverse transcriptase polymerase chain reaction (RT-PCR) of somatostatin receptor subtypes 2, 3, and 5, which have been shown to be involved in tumor regression, might account for the long evolution of this case (>5 yr). This case illustrates the importance of local humoral modulation in tumor growth. Moreover, ultrastructural results favor a unique origin of the tumor cells from one amphicrine cell type.

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Authors and Affiliations

  1. Service d'Anatomie et de Cytologie Pathologiques, Hôpital Lariboisière, 2 rue Ambroise Paré, 75475, Paris cedex 10, France

    Jean-Philippe Brouland MD, PhD, Isabelle Brocheriou-Spelle MD, Michel Wassef MD, Anne Lavergne MD & Jean-Marie Launay PhD

  2. CR C. Bernard “Pathologie Expérimentale et Communications Cellulaires”, 1FR 6, Service de Biochimie, Hôpital Lariboisière AP-HP, 75475, Paris Cédex 10 Paris, France

    Philippe Manivet PhD

  3. Service d'Anatomie Pathologique, Hôpital G. et R. Laennec, B.P. 1005, Nantes, France

    Marie-Françoise Le Bodic MD

Authors
  1. Jean-Philippe Brouland MD, PhD
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  2. Philippe Manivet PhD
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  3. Isabelle Brocheriou-Spelle MD
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  4. Michel Wassef MD
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  5. Marie-Françoise Le Bodic MD
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  6. Anne Lavergne MD
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  7. Jean-Marie Launay PhD
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Correspondence to Jean-Philippe Brouland MD, PhD.

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Brouland, JP., Manivet, P., Brocheriou-Spelle, I. et al. Histological, immunohistochemical, ultrastructural and biochemical study of human gastric composite tumor: Expression of the serotonin-2B receptor by the neuroendocrine component. Endocr Pathol 12, 77–86 (2001). https://doi.org/10.1385/EP:12:1:77

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