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Assessing Growth and Response to Therapy in Murine Tumor Models

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Book cover Chemosensitivity: Volume II

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 111))

Abstract

Rodent models provide an important means of assessing antitumor activity vs toxicity for new cancer therapies. Tumors are often grown subcutaneously on the flank or back of animals, allowing accurate serial determination of tumor volume with calipers by measuring the tumors in three dimensions. The advantages of assessing tumor volume in subcutaneous tumors must be balanced against the potential artifacts induced by growth of tumor cells in subcutaneous tissue. Various orthotopic models have been developed. However, they are more labor-intensive and generally do not allow accurate assessment of tumor growth and/or response unless investigators have access to small animal cross-sectional imaging. Use of small-animal magnetic resonance imaging (MRI) allows one to assess the growth and response of intracavitary tumors, but the cost and labor-intensive nature of MRI limits its use in drug testing. Another approach to intracavitary solid tumor models is the intravenous injection of tumor cells, which can produce lung, liver, or bone metastases (depending on the cell line used), whereas direct injection of tumor cells into the femur or tibia of mice can cause local growth in bone. Progression of both lung metastases and bone lesions can be assessed by small-animal analog X-ray techniques that are more easily available and less labor-intensive to use, and are proving useful for selected therapeutic and biological studies.

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Author information

Authors and Affiliations

  1. USC-CHLA Institute for Pediatric Clinical Research, University of Southern California and Childrens Hospital Los Angeles, Los Angeles, CA, USA

    C. Patrick Reynolds & Bee-Chun Sun

  2. Division of Hematology-Oncology, Keck School Medicine, University Southern California, Los Angeles, CA, USA

    Yves A. DeClerck

  3. Department of Radiology, Keck School Medicine, University Southern California, Los Angeles, CA, USA

    Rex A. Moats

Authors
  1. C. Patrick Reynolds
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  2. Bee-Chun Sun
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  3. Yves A. DeClerck
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  4. Rex A. Moats
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Editors and Affiliations

  1. Garden State Cancer Center, Belleville, NJ, USA

    Rosalyn D. Blumenthal

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© 2005 Humana Press Inc.

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Reynolds, C.P., Sun, BC., DeClerck, Y.A., Moats, R.A. (2005). Assessing Growth and Response to Therapy in Murine Tumor Models. In: Blumenthal, R.D. (eds) Chemosensitivity: Volume II. Methods in Molecular Medicine™, vol 111. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-889-7:335

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  • DOI: https://doi.org/10.1385/1-59259-889-7:335

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-58829-586-6

  • Online ISBN: 978-1-59259-889-2

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