Basic–Alimentary TractA Green Tea Component Suppresses Posttranslational Expression of Basic Fibroblast Growth Factor in Colorectal Cancer
Section snippets
Cell Culture and Reagents
All cell lines were purchased from the American Type Culture Collection (Manassas, VA), except the head and neck cancer cell line, SPCCY-1, which was obtained from Dr Dong M. Shin (Emory University, Atlanta, GA). All chemicals were purchased from Fischer Scientific (Pittsburgh, PA), unless otherwise specified. Primary antibodies for bFGF and β-catenin were purchased from BD Transduction Laboratories (San Jose, CA). VEGF and actin primary antibodies were obtained from Santa Cruz Biotechnology,
Basic FGF Suppression by Green Tea Catechins
Although extensive research has focused on the mechanisms involved in how EGCG affects VEGF suppression,14, 15 little attention has been given to the suppression mechanism of EGCG on bFGF expression. To address this, we examined 4 human colorectal cell lines and found that only 2 cell lines, HCT-116 and LoVo, expressed bFGF. LoVo cells highly expressed bFGF protein, whereas HCT-116 cells marginally expressed bFGF. Notable bFGF was detected as 2 bands (18 and 24 kilodaltons), and EGCG treatment
Discussion
Green tea acts as an anticancer agent, especially in colon cancer. Although the molecular mechanism of this anticancer effect is still not entirely understood, most green tea effects are believed to result from EGCG.23 In addition to its chemopreventive activity, EGCG is known to possess antiangiogenic properties through inhibition of proangiogenic factors including VEGF and bFGF.24, 25 Our results show that EGCG suppresses the protein levels of bFGF and VEGF in colorectal cancer cells, which
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Integrated transcriptomic and metabolomic analyses to characterize the anti-cancer effects of (−)-epigallocatechin-3-gallate in human colon cancer cells
2020, Toxicology and Applied PharmacologyCitation Excerpt :These findings strongly supported a role for glycerophospholipid metabolism as a potentially crucial biological process associated with CRC development and pharmacologic intervention using EGCG. EGCG is established as having significant anti-tumor activity against CRC, and has been identified as a potential therapeutic agent against this type of tumor (Sukhthankar et al., 2008; Adachi et al., 2009). Here, we investigated whether EGCG inhibited proliferation of a CRC cell line: HT-29.
Epigallocatechin-3-gallate (EGCG) inhibits aggregation of pulmonary fibrosis associated mutant surfactant protein A2 via a proteasomal degradation pathway
2019, International Journal of Biochemistry and Cell BiologyA multi-targeted approach to suppress tumor-promoting inflammation
2015, Seminars in Cancer BiologyCitation Excerpt :It promotes pRb hypophosphorylation and activation of this tumor suppressor protein [453], and inhibits MMPs such as MMP-9 [454]. In animal models EGCG prevents the development of adenomatous polyps in ApcMin/+ mice [455,456]. Some epidemiological studies have shown that high consumption of green tea reduces the risk of several types of cancers, including the lung, colorectum, liver, esophagus and stomach [457,458].
Supported primarily by NIH grant R21CA109423 (to S.J.B.) and K26RR016645 (to M.F.M.).
Conflicts of interest: No conflicts of interest exist.