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EPLIN, Epithelial protein lost in neoplasm

Oncogene volume 18, pages 7838–7841 (1999)Cite this article

Abstract

We have identified a novel cytoskeletal protein, EPLIN (Epithelial Protein Lost In Neoplasm), that is preferentially expressed in human epithelial cells. Two EPLIN isoforms, a 600 amino acid EPLIN-α and a 759 amino acid EPLIN-β, are detected in primary epithelial cells of oral mucosa, prostate and mammary glands. The expression of EPLIN-α is either down-regulated or lost in the majority of oral cancer cell lines (8/8), prostate cancer cell lines (4/4) and xenograft tumors (3/3), and breast cancer cell lines (5/6). The amino acid sequence of EPLIN is characterized by the presence of a single centrally located LIM domain. Both EPLIN isoforms localize to filamentous actin and suppress cell proliferation when overexpressed. These findings indicate that the loss of EPLIN seen in cancer cells may play a role in cancer progression.

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Abbreviations

EPLIN:

Epithelial protein lost in neoplasm

MEC:

mammary epithelial cells

IMEC:

immortalized mammary epithelial cells

PrEC:

prostate epithelial cells

NHOK:

normal human oral keratinocytes

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Acknowledgements

We are grateful to M Brown, D Kaetzel, M Navab, N-H Park, M Pegram, R Pietras, R Reiter, R Ross and C Sawyers for providing cell cultures and tissues used in this study. We are also grateful to C Denny, J Gasson, T Lane and M Teitell for comments on the manuscript. This work was supported by National Institutes of Health (GM58215) and Jonsson Cancer Center Foundation (Stop Cancer). RS Maul is supported by NIH Training Grant (T32CA75956).

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  1. Department of Medicine & Micorbiology, Immunology, and Molecular Molecular Genetics, Jonnson Comprehensive Cancer Center, UCLA School of Medicine, Los Angeles, 90095, California, CA, USA

    Raymond S Maul & David D Chang

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  1. Raymond S Maul
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Maul, R., Chang, D. EPLIN, Epithelial protein lost in neoplasm. Oncogene 18, 7838–7841 (1999). https://doi.org/10.1038/sj.onc.1203206

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