Abstract
Membrane-associated guanylate kinase (MAGI)-1/BAI-associated protein (BAP) 1 and Synapse-associated protein (SAP) 97/human Discs-large tumor suppressor gene (hDLG) are ubiquitous isoforms of synaptic scaffolding molecule (S-SCAM) and Postsynaptic density (PSD)-95/SAP90, both of which are implicated in the structures of synapses, respectively. SAP97/hDLG is localized at epithelial junctions and may function as a scaffolding protein, but the subcellular localization or the function of MAGI-1/BAP1 has not been clarified. In intestinal epithelial cells, MAGI-1/BAP1 was localized at tight junctions, whereas SAP97/hDLG was localized diffusely at cell – cell junctions. In Madine Darby canine kidney (MDCK) cells, MAGI-1/BAP1 was colocalized with ZO-1, whereas SAP97/hDLG was colocalized with E-cadherin. In MDCK cells, dominant active and negative mutants of Rac1 small G protein changed the amounts of SAP97/hDLG at cell – cell junctions, but not that of MAGI-1/BAP1. When MDCK cells were switched to a low Ca2+ medium, E-cadherin disappeared from the plasma membrane, and cells were dissociated. The phorbol 12-myristate 13-acetate-treatment after the low Ca2+ switch induced a tight junction-like structure. MAGI-1/BAP1 was recruited with ZO-1 to this structure, but SAP97/hDLG or E-cadherin was not. These findings suggest that MAGI-1/BAP1 is a component of tight junctions of epithelial cells, and that its role is different from that of SAP97/hDLG.
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References
Barth AI, Nathke IS and Nelson WJ. . 1997 Curr. Opin. Cell. Biol. 9: 683–690.
Brose N, Hofmann K, Hata Y and Sudhof TC. . 1995 J. Biol. Chem. 270: 25273–25280.
Cho K-O, Hunt CA and Kennedy MB. . 1992 Neuron 9: 929–942.
Craven SE and Bredt DS. . 1998 Cell 93: 495–498.
Dobrosotskaya I, Guy RK and James GL. . 1997 J. Biol. Chem. 272: 31589–31597.
Gomperts SN. . 1996 Cell 84: 659–662.
Hata Y, Butz S and Sudhof TC. . 1996 J. Neurosci. 16: 2488–2494.
Hata Y, Nakanishi H and Takai Y. . 1998 Neurosci. Res. 32: 1–7.
Hirao K, Hata Y, Ide N, Takeuchi M, Irie M, Yao I, Deguchi M, Toyoda A, Sudhof TC and Takai Y. . 1998 J. Biol. Chem. 273: 21101–21110.
Ide N, Hata Y, Deguchi M, Hirao K, Yao I and Takai Y. . 1999 Biochem. Biophys. Res. Commun. 256: 456–461.
Irie M, Hata Y, Takeuchi M, Ichtchenko K, Toyoda A, Hirao K, Takai Y, Rosahl TW and Sudhof TC. . 1997 Science 277: 1511–1515.
Irie M, Hata Y, Deguchi M, Ide N, Hirao K, Yao I, Nishioka H and Takai Y. . 1999 Oncogene 18: 2811–2817.
Kartenbeck J, Schmelz M, Franke WW and Geiger B. . 1991 J. Cell Biol. 113: 881–892.
Kim E, Naisbitt S, Hsueh Y-P, Rao A, Rothschild A, Craig AM and Sheng M. . 1997 J. Cell Biol. 136: 669–678.
Kistner U, Wenzel BM, Veh RW, Cases-Langhoff C, Garner AM, Appeltauer U, Voss B, Gundelfinger ED and Garner CC. . 1993 J. Biol. Chem. 268: 4580–4583.
Kornau H-C, Schenker LT, Kennedy MB and Seeburg PH. . 1995 Science 269: 1737–1740.
Lue RA, Marfatia SM, Branton D and Chishti AH. . 1994 Proc. Natl. Acad. Sci. USA 91: 9818–9822.
Mandai K, Nakanishi H, Satoh A, Obaishi H, Wada M, Nishioka H, Itoh M, Mizoguchi A, Aoki T, Fujimoto T, Matsuda Y, Tsukita S and Takai Y. . 1997 J. Cell Biol. 139: 517–528.
Matsumine A, Ogai A, Senda T, Okumura N, Satoh K, Baeg GH, Kawahara T, Kobayashi S, Okada M, Toyoshima K and Akiyama T. . 1996 Science 272: 1020–1023.
Muller BM, Kistner U, Veh RW, Cases-Langhoff C, Becker B, Gundelfinger ED and Garner CC. . 1995 J. Neurosci. 15: 2354–2366.
Pawson T and Scott JD. . 1997 Science 278: 2075–2080.
Reuver SM and Garner CC. . 1998 J. Cell Sci. 111: 1071–1080.
Rubinfeld B, Souza B, Albert I, Muller O, Chamberlain SH, Masiarz FR, Munemitsu S and Polakis P. . 1993 Science 262: 1731–1734.
Satoh K, Yanai H, Senda T, Kohu K, Nakamura T, Okumura N, Matsumine A, Kobayashi S, Toyoshima K and Akiyama T. . 1997 Genes Cells 2: 415–424.
Sheng M. . 1996 Neuron 17: 575–578.
Shiratsuchi T, Futamura M, Oda K, Nishimori H, Nakamura Y and Tokino T. . 1998 Biochem. Biophys. Res. Comm. 247: 597–604.
Su LK, Vogelstein B and Kinzler KW. . 1993 Science 262: 1734–1737.
Sudol M. . 1998 Oncogene 17: 1469–1474.
Takaishi K, Sasaki T, Kotani H, Nishioka H and Takai Y. . 1997 J. Cell Biol. 139: 1047–1059.
Takeuchi M, Hata Y, Hirao K, Toyoda A, Irie M and Takai Y. . 1997 J. Biol. Chem. 272: 11943–11951.
Yao I, Hata Y, Ide N, Hirao K, Deguchi M, Nishioka H, Mizoguchi A and Takai Y. . 1999 J. Biol. Chem. 274: 11889–11896.
Acknowledgements
We thank Kenji Takaishi (Osaka University Medical School) for MDCK cells expressing a dominant active or negative Rac1 mutant.
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Ide, N., Hata, Y., Nishioka, H. et al. Localization of membrane-associated guanylate kinase (MAGI)-1/BAI-associated protein (BAP) 1 at tight junctions of epithelial cells. Oncogene 18, 7810–7815 (1999). https://doi.org/10.1038/sj.onc.1203153
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DOI: https://doi.org/10.1038/sj.onc.1203153
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