Abstract
Mycoplasmal contamination of cell culture systems continues to present major problems for basic research and for manufacturing of bioproducts. Previous work suggested that certain antibiotics have strong anti-mycoplasma properties and raised the prospect that the technically rather simple antibiotic treatment may be an appropriate means for mycoplasma eradication. We have developed and validated an effective strategy to eliminate mycoplasma from chronically infected cell cultures using antibiotics which have shown strong activity against these contaminants. Here, we describe our experience with the treatment of 123 consecutive mycoplasma-positive leukemia–lymphoma cell lines, comparing five different antibiotic regimens (in total 433 treatments). We optimized the antibiotic dose schedules and the duration of treatments. The various antibiotic treatments which were employed in parallel had a high efficacy, as 71% to 86% of the infected cultures were cleansed. Treatment failure may result from the resistance of the mycoplasmas to antibiotic therapy and the inability of the eukaryotic cells to survive the cytotoxic effects of the antibiotics. Resistance to mycoplasma eradication was observed in 3% to 20% of the cultures. Loss of the cell culture caused by cytotoxicity was seen in 3% to 11% of the treatments. With regard to the overall outcome, 96% of the cell lines were rendered mycoplasma-free with at least one of the antibiotic treatments and were permanently cured. In conclusion, antibiotic treatment represents the most practical and efficient option to cleanse mycoplasma-positive cell lines.
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References
Hay RJ, Macy ML, Chen TR . Mycoplasma infection of cultured cells Nature 1989 339: 487–488
Drexler HG, Uphoff CC . Contamination of cell culture, mycoplasma. In: Spier E et al (eds) The Encyclopedia of Cell Technology Wiley: New York 2000 pp 609–627
Masters JRW . Human cancer cell lines: fact and fantasy Nature Reviews (Mol Cell Biol) 2000 1: 233–236
Drexler HG (ed). The Leukemia–Lymphoma Cell Line FactsBook Academic Press: San Diego 2000
Fleckenstein E, Uphoff CC, Drexler HG . Effective treatment of mycoplasma contamination in cell lines with enrofloxacin (Baytril) Leukemia 1994 8: 1424–1434
Uphoff CC, Brauer S, Grunicke D, Gignac SM, MacLeod RAF, Quentmeier H, Steube K, Tümmler M, Voges M, Wagner B, Drexler HG . Sensitivity and specificity of five different mycoplasma detection assays Leukemia 1992 6: 335–341
Uphoff CC, Gignac SM, Drexler HG . Mycoplasma contamination in human leukemia cell lines. I. Comparison of various detection methods J Immunol Meth 1992 149: 43–53
Uphoff CC, Drexler HG . Detection of mycoplasma in leukemia–lymphoma cell lines using polymerase chain reaction Leukemia 2002 16: 289–293
Gignac SM, Brauer S, Häne B, Quentmeier H, Drexler HG . Elimination of mycoplasma from infected leukemia cell lines Leukemia 1991 5: 162–165
Uphoff CC, Gignac SM, Drexler HG . Mycoplasma contamination in human leukemia cell lines. II. Elimination with various antibiotics J Immunol Meth 1992 149: 55–62
Barile MF . Mycoplasma-tissue cell interactions. In: Tully JG, Whitcomb RF (eds) The Mycoplasmas, Vol. II Academic Press: New York 1979 pp 425–474
Drexler HG, Dirks WG, MacLeod RAF, Quentmeier H, Steube KG, Uphoff CC (eds). DSMZ Catalogue of Human and Animal Cell Lines, 8th edn Braunschweig, Germany 2001
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Uphoff, C., Meyer, C. & Drexler, H. Elimination of mycoplasma from leukemia–lymphoma cell lines using antibiotics. Leukemia 16, 284–288 (2002). https://doi.org/10.1038/sj.leu.2402364
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DOI: https://doi.org/10.1038/sj.leu.2402364
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