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Gene Therapy Progress and Prospects: Electroporation and other physical methods

Gene Therapy volume 11, pages 1363–1369 (2004)Cite this article

Abstract

Over the last 5 years, physical methods of plasmid delivery have revolutionized the efficiency of nonviral gene transfer, in some cases reaching the efficiencies of viral vectors. In vivo electroporation dramatically increases transfection efficiency for a variety of tissues. Other methods with clinical precedent, pressure-perfusion and ultrasound, also improve plasmid gene transfer. Alternatives such as focused laser, magnetic fields and ballistic (gene gun) approaches can also enhance delivery. As plasmid DNA appears to be a safe gene vector system, it seems likely that plasmid with physically enhanced delivery will be used increasingly in clinical trials.

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  1. Department of Cellular and Molecular Neuroscience, Division of Neuroscience and Psychological Medicine, Gene Targeting Unit, Imperial College London, Charing Cross Hospital, St Dunstan's Road, London, UK

    D J Wells

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Wells, D. Gene Therapy Progress and Prospects: Electroporation and other physical methods. Gene Ther 11, 1363–1369 (2004). https://doi.org/10.1038/sj.gt.3302337

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