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Overexpression of mRNA-binding protein CRD-BP in malignant melanomas

Abstract

Wnt/β-catenin signaling pathway plays an important role in embryogenesis, stem cell maintenance, tumorigenesis and aging. Here, we show that RNA-binding protein, coding region determinant-binding protein (CRD-BP) (a transcriptional target of Wnt signaling pathway), is highly expressed in primary human malignant melanomas and melanoma cell lines with activated Wnt/β-catenin signaling pathway. Upregulation of CRD-BP is associated with an elevated level of β-TrCP1 ubiquitin ligase receptor and activation of nuclear transcriptional factors-kappa B (NF-κB) signaling. Knockdown of CRD-BP inhibits NF-κB activity, induces apoptosis, and suppresses proliferation and tumorigenic properties of melanoma cells.

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Acknowledgements

We thank Drs N Maddodi, S Devi, J Ross, P Robbins, K Kinzler and B Vogelstein for their generous gifts of reagents, S Reagan-Shaw for statistical analysis, Drs V Setaluri and S Fuchs for critical reading of the paper, and Dr K Spiegelman and Ms I Larsen for help with the paper preparation. This study was supported by NCI Grant CA121851 (to VSS).

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Correspondence to V S Spiegelman.

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Supplementary Information accompanies the paper on the Oncogene website (http://www.nature.com/onc)

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Elcheva, I., Tarapore, R., Bhatia, N. et al. Overexpression of mRNA-binding protein CRD-BP in malignant melanomas. Oncogene 27, 5069–5074 (2008). https://doi.org/10.1038/onc.2008.141

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