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Cloning by functional expression of a member of the glutamate receptor family

Nature volume 342, pages 643–648 (1989)Cite this article

Abstract

We have isolated a complementary DNA clone by screening a rat brain cDNA library for expression of kainate-gated ion channels in Xenopus oocytes. The cDNA encodes a single protein of relative molecular mass (Mr) 99,800 which on expression in oocytes forms a functional ion channel possessing the electrophysiological and pharmacological properties of the kainate subtype of the glutamate receptor family in the mammalian central nervous system.

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References

  1. Cajal, S. R. Histologie du Systéme Nerveux de I'Homme et des Vertebrés (Moloine, Paris, 1911).

    Google Scholar 

  2. Hebb, D. O. The Organization of Behavior (Wiley, New York, 1949).

    Google Scholar 

  3. Monaghan, D. T., Bridges, R. J. & Cotman, C. W. A. Rev. Pharmacol. Toxicol. 29, 365–402 (1989).

    Article  CAS  Google Scholar 

  4. Collingridge, G. L. & Bliss, T. V. P. Trends Neurosci. 10, 288–293 (1987).

  5. Foster, A. & Fagg, G. E. Brain Res. Rev. 7, 103–164 (1984).

    Article  CAS  Google Scholar 

  6. Sugiyama, H., Ito, I. & Hirono, C. 325, 531–533 (1987).

  7. Monaghan, D. T., Holets, V. R., Toy, D. W. & Cotman, C. W. Nature 306, 176–179 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  8. McKernan, R. M., Castro, S., Poat, J. A. & Wong, E. H. F. J. Neurochem. 52, 777–785 (1989).

    Article  CAS  PubMed  Google Scholar 

  9. Henley, J. M. & Barnard, E. A. J. Neurochem. 53, 140–148 (1989).

    Article  CAS  PubMed  Google Scholar 

  10. Hampson, D. R. & Wenthold, R. J. J. biol. Chem. 263, 2500–2505 (1988).

    CAS  PubMed  Google Scholar 

  11. Gregor, P., Eshhar, N., Ortega, A. & Teichberg, V. I. EMBO J. 7, 2673–2679 (1988).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Klein, A. U., Niederoest, B., Winterhalter, K. H., Cuenod, M. & Streit, P. Neumsci. Lett. 95, 359–364 (1988).

    Article  CAS  Google Scholar 

  13. Chen, J-W., Cunningham, M. D., Galton, N. & Michaelis, E. K. J. biol. Chem. 263, 417–426 (1989).

    Google Scholar 

  14. Brose, N., Halpain, S., Suchanek, C. & Jahn, R. J. biol. Chem. 264, 9619–9625 (1989).

    CAS  PubMed  Google Scholar 

  15. Masu, Y. et al. Nature 329, 836–838 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  16. Hirono, C., Ito, I., Yamagishi, S. & Sugiyama, H. Neurosci. Res. 6, 106–114 (1988).

    Article  CAS  PubMed  Google Scholar 

  17. Gundersen, C. B., Miledi, R. & Parker, I. Proc. R. Soc.B221, 127–143 (1984).

    ADS  CAS  Google Scholar 

  18. Lerma, J., Kushner, L., Spray, D. C., Bennett, M. V. L. & Zukin, R. S. Proc. natn. Acad. Sci. U.S.A 86, 2083–2087 (1989).

    Article  ADS  CAS  Google Scholar 

  19. Verdoorn, T. A. & Dingledine, R. Molec. Pharmacol. 34, 298–307 (1988).

    CAS  Google Scholar 

  20. Davies, J. & Watkins, J. C. Brain Res. 206, 172–177 (1981).

    Article  CAS  PubMed  Google Scholar 

  21. Fagg, G. E. Trends Neurosci. 8, 207–210 (1985).

    Article  CAS  Google Scholar 

  22. von Heijne, G. Nucleic Acids Res. 14, 4683–4690 (1986).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Grenningloh, G. et al. Nature 330, 25–26 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  24. Barnard, E. A., Darlison, M. G. & Seeburg, P. Trends Neurosci. 10, 502–509 (1987).

    Article  CAS  Google Scholar 

  25. Feng, D. F., Johnson, M. S. & Doolittle, R. F. J. molec. Evol. 21, 112–125 (1985).

    Article  ADS  CAS  Google Scholar 

  26. Deneris, E. S., Boulter, J., Swanson, L. W., Patrick, J. & Heinemann, S. J. J. biol. Chem. 264, 6268–6272 (1989).

    CAS  PubMed  Google Scholar 

  27. Duvoisin, R., Deneris, E. S., Patrick, J. & Heinemann, S. Neuron 3, 487–496 (1989).

    Article  CAS  PubMed  Google Scholar 

  28. Schofield, P. R. et al. Nature 328, 221–227 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  29. Grenningloh, G. et al. Nature 328, 215–220 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  30. Giraudat, J., Dennis, M., Heidmann, T., Chang, J. Y. & Changeaux, J. P. Proc. natn. Acad. Sci. U.S.A. 83, 2719–2723 (1986).

    Article  ADS  CAS  Google Scholar 

  31. Patel, S., Meldrum, B. S. & Collins, J. F. Neurosci. Lett. 70, 301–307 (1986).

    Article  CAS  PubMed  Google Scholar 

  32. Perouansky, M. & Grantyn, R. J. Neurosci. 9, 70–80 (1989).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Boulter, J. et al. Proc. natn. Acad. Sci. U.S.A. 84, 7763–7767 (1987).

    Article  ADS  CAS  Google Scholar 

  34. Hampson, D. R., Wheaton, K. D., Dechesne, C. J. & Wenthold, R. J. J. biol. Chem. 264, 13329–13335 (1989).

    CAS  PubMed  Google Scholar 

  35. Blair, L. A. C., Levitan, E. S., Marshall, J., Dionne, V. E. & Barnard, E. A. Science 242, 577–579 (1988).

    Article  ADS  CAS  PubMed  Google Scholar 

  36. Shivers, B. D. et al. Neuron 3, 327–337 (1989).

    Article  CAS  PubMed  Google Scholar 

  37. Schmieden, V., Grenningloh, G., Schofield, P. R. & Betz, H. EMBO J. 8, 695–700 (1989).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Pritchet, D. B., Lüddens, H. & Seeburg, P. H. Science 245, 1389–1392 (1989).

    Article  ADS  Google Scholar 

  39. Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J. & Rutter, W. J. Biochem. 18, 5294–5299 (1979).

    Article  CAS  Google Scholar 

  40. Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).

    Article  ADS  CAS  Google Scholar 

  41. Short, J. M., Fernandez, J. M., Sorge, J. A. & Huse, W. D. Nucleic Acids Res. 16, 7583–7600 (1988).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  42. Messing, J., Gronenborn, B., Müller-Hill, B. & Hofschneider, P. H. Proc. natn. Acad. Sci. U.S.A. 74, 3642–3646 (1977).

    Article  ADS  CAS  Google Scholar 

  43. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  44. Kyte, J. & Doolittle, R. F. J. molec. Biol. 157, 105–132 (1982).

    Article  CAS  PubMed  Google Scholar 

  45. Devereux, J., Haeberli, P. & Smithies, O. Nucleic Acids Res. 12, 387–395 (1984).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  46. Heinemann, S. et al. in Molecular Neurobiology: Recombinant DNA Approaches (eds Heinemann, S. & Patrick, J.) 45–96 (Plenum, New York, 1987).

    Google Scholar 

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Authors and Affiliations

  1. Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, California, 92037, USA

    Michael Hollmann, Anne O'Shea-Greenfield, Scott W. Rogers & Stephen Heinemann

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  1. Michael Hollmann
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  2. Anne O'Shea-Greenfield
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  3. Scott W. Rogers
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  4. Stephen Heinemann
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Hollmann, M., O'Shea-Greenfield, A., Rogers, S. et al. Cloning by functional expression of a member of the glutamate receptor family. Nature 342, 643–648 (1989). https://doi.org/10.1038/342643a0

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