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ICAM-1 supports adhesion of human small-cell lung carcinoma to endothelial cells

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Abstract

Adhesion of tumor cells to endothelium via cell-adhesion molecules constitutes a crucial step in metastasis, which is largely responsible for the poor prognosis of small-cell lung carcinoma (SCLC). Patients with SCLC were reported to have elevated levels of intercellular adhesion molecule-1 (ICAM-1). The present study therefore focuses on endothelial ICAM-1 in tumor-cell adhesion. We found that the adherence of SCLC cells (cell lines H24, H69, H82) to cultured vascular endothelium in stasis and flow depends on the expression of ICAM-1. After blocking endothelial ICAM-1 with monoclonal antibodies, adhesion was significantly reduced. These results pinpoint ICAM-1 for the first time as a molecule crucially involved in SCLC cell-endothelial adhesion.

Abbreviations: ECGS – endothelial cell growth supplement; FCS – fetal calf serum; HUVECs – human umbilical vein endothelial cells; ICAM-1 – intercellular adhesion molecule-1; IL-1β– Interleukin-1β; SCLC – small-cell lung carcinoma

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References

  1. Carney DN. Lung cancer-time to move on from chemotherapy. N Engl J Med 2002; 346: 126–8.

    Article  PubMed  Google Scholar 

  2. Orr FW, Wang HH, Lafrenie RM et al. Interactions between cancer cells and the endothelium in metastasis. J Pathol 2000; 190: 310–29.

    Article  PubMed  CAS  Google Scholar 

  3. Taguchi O, Gabazza EC, Kobayashi T et al. Circulating intercellular adhesion molecule-1 in patients with lung cancer. Intern Med 1997; 36: 14–8.

    PubMed  CAS  Google Scholar 

  4. Jaffe EA, Nachmann RL, Becker cg et al. Culture of human endothelial cells derived from umbilical veins: Identification of morphology and immunologic criteria. J Clin Invest 1973; 52: 2745–56.

    PubMed  CAS  Google Scholar 

  5. Aletsee-Ufrecht MC, Langley K, Rotsch m et al. NCAM: A surface marker for human small cell lung cancer cells. FEBS Lett 1990; 267: 295–300.

    Article  PubMed  CAS  Google Scholar 

  6. Reininger AJ, Korndorfer MA, Wurzinger LJ. Adhesion of ADPactivated platelets to intact endothelium under stagnation point flow in vitro is mediated by the integrin aIIbβ3. Thromb Haemost 1998; 79: 998–1003.

    PubMed  CAS  Google Scholar 

  7. Zerwes HG, Peter JC, Link m et al. A multiparameter screening assay to assess the cytokine-induced expression of endothelial cell adhesion molecules. Anal Biochem 2002; 304: 166–73.

    Article  PubMed  CAS  Google Scholar 

  8. Neelamegham S, Taylor AD, Burns AR et al. Hydrodynamic shear shows distinct roles for LFA-1 and Mac-1 in neutrophil adhesion to intercellular adhesion molecule-1. Blood 1998; 92: 1626–38.

    PubMed  CAS  Google Scholar 

  9. Smith CW, Marlin SD, Rothlein R et al. Cooperative interactions of LFA-1 and Mac-1 with intercellular adhesion molecule-1 in facilitating adherence and transendothelial migration of human neutrophils in vitro. J Clin Invest 1989; 83: 2008–17.

    Article  PubMed  CAS  Google Scholar 

  10. Grander D, Xu B, Wang P et al. Gamma-IFN induced cell adhesion in chronic myelogenous leukemia cells. Leukemia 1994; 8: 299–304.

    PubMed  CAS  Google Scholar 

  11. Stoelcker B, Hafner m, Orosz P et al. Role of adhesion molecules and platelets in TNF-induced adhesion of tumor cells to endothelial cells: implications for experimental metastasis. J Inflam 1996; 46: 155–67.

    CAS  Google Scholar 

  12. Wang HH, Nance DM, Orr W.Murine hepatic microvascular adhesion molecule expression is inducible and has a zonal distribution. Clin Exp Metast 1999; 17: 149–55.

    Article  CAS  Google Scholar 

  13. Hakomori S. Novel endothelial cell activation factor(s) released from activated platelets which induce E-selectin expression and tumor cell adhesion to endothelial cells: A preliminary note. Biochem Biophys Res Commun 1994; 203: 1605–13.

    Google Scholar 

  14. Feldman LE, Shin KC, Natale RB et al.β 1 integrin expression on human small cell lung cancer cells. Cancer Res 1991; 51: 1065–70.

    PubMed  CAS  Google Scholar 

  15. Pottratz ST, Hall TD, Scribner WM et al. P-selectin-mediated attachment of small cell lung carcinoma to endothelial cells. Am J Physiol 1996; 271: L918–L23.

    PubMed  CAS  Google Scholar 

  16. Sheski FD, Natarajan V, Pottratz ST. Tumor necrosis factor-a stimulates attachment of small cell lung carcinoma to endothelial cells. J Lab Clin Med 1999; 133: 265–73.

    Article  PubMed  CAS  Google Scholar 

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Authors
  1. Angela H. Finzel
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  2. Armin J. Reininger
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  3. Patrick A. Bode
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  4. Laurenz J. Wurzinger
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Finzel, A.H., Reininger, A.J., Bode, P.A. et al. ICAM-1 supports adhesion of human small-cell lung carcinoma to endothelial cells. Clin Exp Metastasis 21, 185–189 (2004). https://doi.org/10.1023/B:CLIN.0000037696.36108.27

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  • DOI: https://doi.org/10.1023/B:CLIN.0000037696.36108.27

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