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Bifidobacterium longum as a delivery system for gene therapy of chemically induced rat mammary tumors

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Abstract

A fundamental obstacle in cancer gene therapy is the specific targeting of therapy directly to a solid tumor, and no systemic delivery system yet exists. A strain of domestic bacteria, Bifidobacteriumlongum, which is nonpathogenic and anaerobic, selectively localized to and proliferated in 7,12‐dimethylbenz[a]anthracene‐induced rat mammary tumors after systemic application. We further ascertained the tumor specificity of genetically engineered, as well as wild‐type, Bifidobacterium longum. This is the first demonstration that Bifidobacterium longum can be utilized as a specific gene delivery vector for gene therapy on solid breast tumors.

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Authors and Affiliations

  1. Departments of Surgery, Shinshu University School of Medicine, Asahi 3‐1‐1, Matsumoto, 390‐8621, Japan

    Kazuyuki Yazawa, Minoru Fujimori, Toshiyuki Nakamura, Takayuki Sasaki & Jun Amano

  2. Department of Molecular Genetics, Institute of Molecular and Cellular Biology for Pharmaceutical Sciences, Kyoto Pharmaceutical University, 1 Shichono‐cho, Misasagi, Yamashina‐ku, Kyoto, 607‐8412, Japan

    Yasunobu Kano

  3. Molecular Oncology and Angiology, Angio‐Aging Research Division, Research Center on Aging and Adaptation, Shinshu University School of Medicine, Asahi 3‐1‐1, Matsumoto, 390‐8621, Japan

    Shun’ichiro Taniguchi

Authors
  1. Kazuyuki Yazawa
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  2. Minoru Fujimori
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  3. Toshiyuki Nakamura
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  4. Takayuki Sasaki
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  5. Jun Amano
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  6. Yasunobu Kano
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  7. Shun’ichiro Taniguchi
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Yazawa, K., Fujimori, M., Nakamura, T. et al. Bifidobacterium longum as a delivery system for gene therapy of chemically induced rat mammary tumors. Breast Cancer Res Treat 66, 165–170 (2001). https://doi.org/10.1023/A:1010644217648

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  • DOI: https://doi.org/10.1023/A:1010644217648

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