Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology
EndodontologyThe induction of cytotoxicity, oxidative stress, and genotoxicity by root canal sealers in mammalian cells
Section snippets
Preparation of extracts
Four materials were included in this study: AH Plus (Dentsply Maillefer, Konstanz, Germany); Epiphany (Pentron Clinical, Wallingford, CT); Acroseal (Septodont, Saint-Maur-des-Fossés, France); and COP (Polifil; Poliquil Araraquara Polímeros Químicos, Araraquara, Brazil). Polifil is a material which consists of a liquid (polymer extracted from oil of Ricinus communis), a paste (polyester), and zinc oxide as described recently.5 Specifications of the endodontic sealers tested are listed in Table I.
Cytotoxicity of endodontic sealers
All sealers tested cytotoxic under the experimental conditions except for COP, but the extent of effects varied between the materials, as indicated by the differences in the dose-response relationship (Fig. 1). Acroseal was found to be the most cytotoxic material, because a 1:4 dilution of its original extracts reduced cell numbers to 5% compared with untreated negative control cultures. The slight increase in optical density readings detected in cell cultures exposed to higher-concentrated
Discussion
The scientific value of the in vitro evaluation is beyond doubt according to our current understanding of the preclinical testing of the biocompatibility of dental materials. The aim of the use of in vitro cell culture techniques is to study toxicity but not tissue response. Tissue reaction is a result of several factors, among which toxicity is only one. Yet, one can predict that a considerably toxic material will cause severe irritation to tissues in vivo.25 It has also been suggested that
References (37)
- et al.
Comparability of results from two leakage models
Oral Surg Oral Med Oral Pathol Oral Radiol Endod
(2008) - et al.
Initial in vitro biological response to contemporary endodontic sealers
J Endod
(2006) - et al.
In vitro cytotoxicity of a new epoxy resin root canal sealer
J Endod
(2000) - et al.
Mutagenicity of AH26 in an in vitro mammalian cell mutation assay
J Endod
(1995) - et al.
Cytotoxic and mutagenic potencies of various root canal filling materials in eukaryotic and prokaryotic cells in vitro
J Endod
(1999) - et al.
Stimulation of glutathione depletion, ROS production and cell cycle arrest of dental pulp cells and gingival epithelial cells by HEMA
Biomaterials
(2005) - et al.
Inhibition of TEGDMA and HEMA-induced genotoxicity and cell cycle arrest by N-acetylcysteine
Dent Mater
(2007) - et al.
Mechanisms of root canal sealers cytotoxicity on osteoblastic cell line MC3T3-E1
Oral Surg Oral Med Oral Pathol Oral Radiol Endod
(2007) - et al.
The induction of oxidative stress, cytotoxicity, and genotoxicity by dental adhesives
Dent Mater
(2008) - et al.
The induction of micronuclei in V79 cells by the root canal filling material AH Plus
Biomaterials
(2000)
Correlation of in vivo and in vitro screening tests
J Endod
Correlation of screening tests to usage tests
J Endod
The cytotoxicity of RoekoSeal and AH Plus compared during different setting periods
J Endod
The biocompatibility evaluation of epoxy resin-based root canal sealers in vitro
Biomaterials
Setting times of resilon and other sealers in aerobic and anaerobic environments
J Endod
In vitro micronucleus assay with Chinese hamster V79 cells—results of a collaborative study with in situ exposure to 26 chemical substances
Mutat Res
Biocompatibility of root canal filling materials
Aust Endod J
Cytotoxicity of new resin calcium hydroxide and silicone-based root canal sealers on fibroblasts derived from human gingiva and L929 cell lines
Int Endod J
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2024, Saudi Dental JournalEffect of Resin-based and Bioceramic Root Canal Sealers on Postoperative Pain: A Split-mouth Randomized Controlled Trial
2018, Journal of EndodonticsCitation Excerpt :Oxidative stress and, more specifically, ROS have been shown to be linked with inflammatory pain in vivo (16, 17). The production of ROS increased 4 to 7 times when the human pulp cells had been treated with the root canal sealers in vitro (18). Resin-based AH Plus was slightly cytotoxic (10) and released toxic monomers, such as bisphenol A diglicidyl ether (19).
Comparison of antimicrobial activity of traditional and new developed root sealers against pathogens related root canal
2018, Journal of Dental SciencesCitation Excerpt :According to the results of this study, traditional sealers may be effective in treating primary root canal infections, whereas Endoseal, a calcium silicate-base sealer that contains oxide compounds, is more effective in preventing re-infection with E. faecalis. However, considering that traditional sealers have a cytotoxic effect on human pulp cell in vitro.17,24,25 Endoseal may be useful for preventing bacterial infection in untreated and treated root canals.
Cytotoxicity and genotoxicity of thymol verified in murine macrophages (RAW 264.7) after antimicrobial analysis in Candida albicans, Staphylococcus aureus, and Streptococcus mutans
2018, Journal of Functional FoodsCitation Excerpt :The OD obtained at 450 nm in a spectrophotometer were converted in picograms per milliliter (pg/mL), considering the standard curve of TNF-α, using GraphPad Prism 5.0 software for this. This analysis was performed according to Camargo et al. (2009). On sterile glass slides in sterile polystyrene chamber with four compartments (Nunc, Roskilde, Denmark) 4000 μL of DMEM containing 3 × 105 viable cells were added.
Supported by the Department of Operative Dentistry and Periodontology, University of Regensburg, Germany, and the State of São Paulo Research Foundation (FAPESP), São Paulo, Brazil—process no. 07.1744-2.