Fascin Protrusions in Cell Interactions
Section snippets
Roles of Cell Protrusions in Cell Interactions
Cell protrusions are outward extensions of the plasma membrane of individual cells. They are characteristic of animal and ameboid cells, in which the plasma membrane is not limited by a cell wall. By extending outward into the local environment, cell protrusions make initial adhesions to extracellular matrix (ECM) or other cells and sense and interact with the cell environment. Cues received are relayed back to the cell body by cell-signaling mechanisms and influence subsequent cell behavior:
Structure and Binding Properties
Fascin was discovered as a 55-kDa, actin-bundling protein in extracts of sea urchin oocytes and coelomocytes. Subsequent cDNA sequence information revealed this protein to be related to the Drosophila melanogaster singed protein and a human 55-kDa, actin-bundling protein (Bryan et al., 1993, Mosialos et al., 1994). Available complete genome sequences now clarify that a single form of fascin is encoded in the D. melanogaster (Adams et al. 2000), Anopheles gambiae (EAA12061; Holt et al. 2002),
Roles of Fascin Protrusions in the Cardiovascular System in Health and Disease
Many studies of the cellular roles of fascin-1 have relied on myoblasts, neurons, and fibroblasts. However, there are multiple cell types within the cardiovascular system that express fascin-1, as has been documented by immunohistochemistry (Table 1).
Prospective Future Directions
There is considerable cellular and molecular evidence that fascin-based protrusions are important in cell–cell and cell–ECM interactions and contribute to cell migration. The binding of fascin-1 to F-actin is controlled by multiple points of regulation that ensure that actin-bundling activity is made responsive to extracellular cues and occurs appropriately in space and time. We need to understand more of the molecular mechanisms by which diverse extracellular cues—acting through many
Acknowledgements
This research was supported by National Institutes of Health grant GM068073. The author apologizes that not all the primary literature could be cited due to space limitations.
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2021, Molecular Therapy OncolyticsCitation Excerpt :FSCN1 is an actin-bundling protein that organizes actin filaments into parallel bundles. Therefore, it is involved in a broad range of cellular physiological processes, including regulation of cell adhesion, motility, migration, and cellular interactions.2,42,43 In addition, FSCN1 regulates focal adhesion dynamics,44 cell migration and invasion,36,38,45 histone methylation and gene transcription,46 extracellular vesicle release,47 and cancer cell stemness,38,48,49 independently of its actin-bundling activity.
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2018, Biochemical and Biophysical Research CommunicationsCitation Excerpt :Fascin-1 is a cytoskeletal protein with a relative molecular mass of 55,000 Da [5] and it can specifically bind to F-actin [5]. The fascin-1 gene is located on chromosome 7 q22 [6]. Fascin-1 is usually expressed in neuronal, stromal and endothelial cells, but not in epithelial cells [7].
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