Original CommunicationPostoperative intra-abdominal infection increases angiogenesis and tumor recurrence after surgical excision of colon cancer in mice
Section snippets
Animals
A total of 70 Balb/c mice (Harlan Interfauna Iberica, Barcelona, Spain), 6–8 weeks of age, weighing 19–21 g, were used for the experiments. The animals were allowed free access to a standard laboratory diet and water. All procedures were reviewed and approved by the IMIM-Hospital del Mar Animal Care and Use Committee in accordance with European guidelines.
Tumor cell line
A colonic adenocarcinoma cell line (B51LiM), obtained from Robert S. Bresalier, MD, at the Henry Ford Health Sciences Center, Detroit, MI, was
Operative findings and postoperative mortality
After randomization of the 70 mice, 28 were assigned to group 1 and 42 to group 2. Of the 70 mice, 11 died within 48 hours after the cecal injection (3 in group 1 and 8 in group 2); in most animals, the cause of death was cecal ischemia. Another 10 animals (14%) were excluded from the study at the time of cecal resection: in 5 of these 10 animals, there was no tumor in the cecum; in the other 5 animals, there were peritoneal implants, probably because of the spillage of cells during the cecal
Discussion
Several cohort and case-control studies have shown that postoperative intra-abdominal infection is associated with greater rates of tumor recurrence and cancer-specific mortality after potentially curative resection for colorectal cancer.5, 6, 7, 8, 9, 10, 11, 12, 13, 14 In this report, we provide direct evidence pointing to a causative role of intra-abdominal infection in the recurrence of resected cecal tumors by using the in vivo B51LiM colon cancer mouse model and postoperative infection
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Supported by a research grant from the Asociación Española Contra el Cáncer (No. 449).