Central Surgical AssociationSurvivin, a potential biomarker in the development of Barrett's adenocarcinoma
Section snippets
Tissue samples for semiquantitative real-time PCR
Tissue samples (n = 142) were obtained from endoscopy or operative specimens from 100 patients with foregut symptoms and were snap-frozen immediately in liquid nitrogen. Samples containing Barrett's metaplasia, dysplasia (high- or low-grade), or cancer were separated into 3 different groups based on routine histology: (1) Barrett's group: 23 samples from 16 patients showing intestinal metaplasia; (2) dysplasia group: 18 samples from 12 patients showing intestinal metaplasia and either low- (n =
Results
The study population included 29 women and 71 men. The median age of the patients was 57 years (range, 23-86 y). The median value and range of expression levels of survivin determined by semiquantitative reverse-transcription PCR analysis of the 5 tissue groups are listed in Table II (142 tissue samples from 100 patients) and shown in the figure.
Survivin gene expression levels differed among all histologic groups analyzed (P < .001). Survivin mRNA levels were lowest in squamous epithelium from
Discussion
The balance between cell division and cell loss is important for the maintenance of normal human tissue. An imbalance promoting either increased proliferation and/or decreased cell loss results in the uncontrolled cell growth characteristic of carcinogenesis. Recent studies have shown that the deregulation of apoptosis is an essential event in the development of Barrett's-associated adenocarcinoma and that the anti-apoptotic gene survivin is upregulated in esophageal cancer.3, 6, 7, 8, 9, 10
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Cited by (25)
Therapeutic strategies for esophagogastric junction cancer
2015, Formosan Journal of SurgeryCitation Excerpt :The predictive value of EGFR and HER-2/NEU messenger RNA (mRNA) expression in E (Ade) tumors after neoadjuvant CRT with cisplatin and 5-FU followed by transthoracic esophagectomy is not associated with the degree of histopathological response; the low intratumoral expression levels of HER-2/NEU are significantly associated with pCR.73 The roles of p53 and survivin mRNA as predictive markers of pCR in patients with locally advanced EGJ cancer who received trimodality therapy are minor and inconsistent.74 Cyclooxygenase-2 (COX-2) is involved in the regulation of tumor progression, angiogenesis, and resistance to chemotherapy.
Loss of p53, rather than beta-catenin overexpression, induces survivin-mediated resistance to apoptosis in an esophageal cancer cell line
2010, Journal of Thoracic and Cardiovascular SurgeryCitation Excerpt :However, overexpression of wild-type p53 in esophageal cancer cells had no effect on survivin protein levels (Figure 5, C), suggesting a posttranscriptional control point that enhances survivin gene expression. Overexpression of survivin is observed in many types of human cancer, including esophageal cancer,9–11 and is associated with aberrant progression of transformed cells through mitosis.8 The results reported in this study clearly demonstrate that overexpression of survivin plays an important role in the resistance to apoptosis observed in TE7 cells.
Towards the molecular characterization of disease: Comparison of molecular and histological analysis of esophageal epithelia
2007, Journal of Gastrointestinal SurgerySurvivin expression in oesophageal squamous cell carcinoma: its prognostic impact and splice variant expression
2010, European Journal of Cardio-thoracic SurgeryBiology of esophageal cancer: Mechanisms of resistance and metastases
2023, New Research On Esophageal Cancer: Horizons In Cancer Research
Presented at the 62nd Annual Meeting of the Central Surgical Association, Tucson, Arizona, March 10-15, 2005.
Supported by National Institutes of Health grants RO1-CA84424-02.