Original ArticleProteomics-based identification of spontaneous regression-associated proteins in neuroblastoma
Section snippets
Patients and clinical samples
Tumor tissue samples from 8 patients who were all younger than 1 year and had been diagnosed with neuroblastoma were obtained from the Beijing Children's Hospital and China PLA General Hospital. Among these cases, 4 were selected as stage 4 neuroblastoma and the other 4 as stage 4s neuroblastoma. The distinction of tumor staging was based on the International Neuroblastoma Staging System. Tumor resections were performed after valid informed consent had been taken. The samples were collected at
Separation of total proteins by 2D-DIGE
Although the internal standard was incorporated into the 2D-DIGE experiment to eliminate the gel-to-gel variation, good reproducibility among different gels is the basis for effective differential protein expression analysis. The Cy2-labeled gel images of the 4 gels on which the same internal standard was loaded in equal amounts displayed a good reproducibility among different gels and was significantly elevated overall in our experiments.
The 3 cyanine dye images for each gel were overlaid.
Discussion
The mechanism for spontaneous regression of neuroblastomas is still poorly understood. Some researchers believed that neuroblastoma tissue was derived from a malignant transformation of degenerated embryonic tissue; therefore, its spontaneous regression might be, in fact, regression of the embryo. Recently, some groups found morphologic features of apoptosis in stage 4s neuroblastomas, which led to the assumption that apoptosis, which is a representative form of programmed cell death (PCD),
Acknowledgments
This research work was fully supported by a grant from the Chinese Natural Science Foundation, Permit No. 30772275, and a grant from China Project 863, Permit No. 2007AA021004.
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