Basic ResearchCytotoxicity and Genotoxicity of Root Canal Sealers Based on Mineral Trioxide Aggregate
Section snippets
Preparation of Extracts
The following materials were used: white MTA (MTA Branco; Angelus), MTA Fillapex (Angelus), and AH Plus (Dentsply). The main components list of tested material is shown in Table 1. The sealer samples were prepared in 24-well plates (16.2 mm in diameter, 2 mm high), and they were incubated at 37°C for 12, 48, or 72 hours immediately after mixing. Specimens were then covered with 2.5 mL cell culture Dulbecco modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum, penicillin, and
Cytotoxicity of Sealers
The results obtained from a spectrophotometer indicate the activity of cellular metabolism. They represent the inhibition of succinyl dehydrogenase activity, which is caused by the contact between cells and sealer extracts serially diluted to 1:1 (original extract), 1:2, 1:4, 1:8, 1:16, and 1:32. The percentage of cell viability was considered optimal when the average value obtained was 50% or higher. It could be observed by the cell survival mean values that White MTA kept cell viability rates
Discussion
An MTA-based sealer (MTA Fillapex) was recently released to be used in root canal filling. However, when analyzing the chemical composition supplied by the manufacturer, it is worth emphasizing that this is a resin cement containing MTA. For that reason, it was expected that Fillapex MTA acted as a repairer.
In our study, the biocompatibility of MTA repairer (White MTA) and MTA sealer (Fillapex MTA) was assessed by the MTT cytotoxicity test that determines the number of viable cells as a
Conclusions
White MTA cement kept the rate of cell viability above 70% for all dilutions and all the assessed periods. MTA Fillapex sealer was cytotoxic at the dilutions 1:1, 1:2, 1:4, and 1:8 (12-hour group); it showed the highest cytotoxicity in this study. AH Plus was cytotoxic at the dilutions 1:1, 1:2, and 1:4 (48-hour group). White MTA cement stimulated the lower rates of micronuclei formation, even in the highest dilution (1:1); MTA Fillapex and AH Plus stimulated the formation of micronuclei to
Acknowledgments
The authors deny any conflicts of interest related to this study.
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This study was supported by CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil.