Original contributionsDysregulated expression of S100A11 (calgizzarin) in prostate cancer and precursor lesions☆
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Patients and prostate tissue
Prostate tissue from 88 patients attending 2 centers (Academic Urology Unit, University of Sheffield and CeRePP-EA3104, Université Paris 7), were used after full informed consent and local ethics committee approval. These comprised 30 paraffin-embedded cases of benign prostatic hyperplasia (BPH), which were obtained by transurethral resection of prostate, and 54 cases of prostatic adenocarcinoma with matching adjacent benign epithelium, which were obtained either by radical prostatectomy or
Specificity of the anti-S100A11 antibody
The specificity of the guinea pig anti-S100A11 antibody has been reported previously and has been shown to be highly reactive against human recombinant S100A11 but unreactive against recombinant S100A1, S100B, or S100A6 proteins.24 Western blot analysis of prostate cancer cell lines, LNCaP, Du145, and PC3 showed the presence of a band at the expected size of ∼10 KDa, which corresponded to the band seen by using recombinant S100A11 protein (Fig 2). Preimmune guinea-pig serum used as a negative
Discussion
By using immunohistochemistry, we have shown that in benign prostate epithelium, significantly higher expression of S100A11 occurs within the basal cells compared with the adjacent luminal cells. The relationship between basal and luminal cells in prostatic epithelium is poorly understood.26 One hypothesis proposes that the terminally differentiated luminal cells are derived from the basal cell layer via a process involving cell division and differentiation, whereas another suggests that basal
Acknowledgements
The authors thank Dr Sabapathy Balasubramanian for advice and help with the statistical analysis.
References (33)
- et al.
Expression analysis of S100 proteins and RAGE in human tumors using tissue microarrays
Biochem Biophys Res Commun
(2003) - et al.
Purification, characterization, and partial sequence analysis of a newly identified EF-hand type 13-kDa Ca(2+)-binding protein from smooth muscle and non-muscle tissues
J Biol Chem
(1991) - et al.
Human S100A11 exhibits differential steady-state RNA levels in various tissues and a distinct subcellular localization
Biochem Biophys Res Commun
(1999) - et al.
Human calgizzarin; one colorectal cancer-related gene selected by a large scale random cDNA sequencing and northern blot analysis
Cancer Lett
(1995) - et al.
Molecular cloning and sequencing of a cDNA clone encoding a new calcium binding protein, named calgizzarin, from rabbit lung
Biochem Biophys Res Commun
(1991) - et al.
Identification of genetic markers for prostatic cancer progression
Lab Invest
(2000) - et al.
Genetic abnormalities specifically associated with varying metastatic potential of prostate cancer cell lines as detected by comparative genomic hybridization
Cancer Genet Cytogenet
(2001) - et al.
S100A6 and S100A11 are specific targets of the calcium- and zinc-binding S100B protein in vivo
J Biol Chem
(2000) Intracellular and extracellular roles of S100 proteins
Microsc Res Technol
(2003)- et al.
S100 proteinsStructure, functions and pathology
Front Biosci
(2002)
Psoriasin (S100A7) expression is associated with poor outcome in estrogen receptor-negative invasive breast cancer
Clin Cancer Res
S100A4 regulates cell motility and invasion in an in vitro model for breast cancer metastasis
Br J Cancer
Differential expression of S100A2 and S100A4 during progression of human prostate adenocarcinoma
J Clin Oncol
Localization of S100C immunoreactivity in various human tissues
Acta Med Okayama
Molecular alterations in pancreatic carcinomaExpression profiling shows that dysregulated expression of S100 genes is highly prevalent
J Pathol
Increased expression of S100A6 at the invading fronts of the primary lesion and liver metastasis in patients with colorectal adenocarcinoma
Br J Cancer
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2021, Cellular and Molecular Gastroenterology and HepatologyCitation Excerpt :NAFLD is a severe liver disease, and numerous factors contribute to its development. Although some studies have reported that S100A11 is an important factor in diseases such as cancer35,36 and rheumatoid arthritis,55 its role in lipid metabolism has not yet been addressed. Several lines of evidence, including ours, convincingly support that S100A11 is a pathogenic factor of NAFLD.
A review of S100 protein family in lung cancer
2018, Clinica Chimica ActaCitation Excerpt :From then on, an endless stream of studies regarding to this protein family have appeared and the expression of various S100 proteins has been demonstrated in a diverse spectrum of tissues [6,7]. In recent years, there is an increasing interest in S100 protein family since it has been verified to have a close relationship with tumorigenesis and many members have abnormal expressions in numerous human cancers [8–26]. Meanwhile, lung cancer, which is the leading worldwide cause of tumor-related deaths, has also been shown to be relevant to S100 proteins [27–46].
Annexins are instrumental for efficient plasma membrane repair in cancer cells
2015, Seminars in Cell and Developmental BiologyCitation Excerpt :These proteins and other Annexin and S100 family members in general are frequently upregulated in a variety of cancers [64,65]. Both S100A11 and ANXA2 are overexpressed in several cancer types, where it is associated with metastasis and poor overall survival [66–69]. Furthermore, S100A11 is enriched in pseudopodia of metastatic cancer cells and it is implicated in forming actin-dependent pseudopodial protrusions, which is required for tumor cell migration [70].
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I.R. is supported by an NCRI Collaborative grant to FCH (MRC 58152).